Jo-1, Thymus Enzyme
Jo-1, Thymus (Histidyl t-RNA Synthetase)
Applications
ELISA
Purity
Affinity PurifiedPurified from calf and/or rabbit thymus through the use of immobilized human anti-Jo-1 immune globulins. Other antigens (Sm, RNP, SSA, Scl-70, and SSB) are not detected with ELISA assays. Homogeneity is verified by SDS PAGE.
Synonyms
Jo-1; Thymus; Thymus (Histidyl t-RNA Synthetase); Thymus enzyme
Host
Calf Thymus
Purity/Purification
Affinity Purified
Purified from calf and/or rabbit thymus through the use of immobilized human anti-Jo-1 immune globulins. Other antigens (Sm, RNP, SSA, Scl-70, and SSB) are not detected with ELISA assays. Homogeneity is verified by SDS PAGE.
Purified from calf and/or rabbit thymus through the use of immobilized human anti-Jo-1 immune globulins. Other antigens (Sm, RNP, SSA, Scl-70, and SSB) are not detected with ELISA assays. Homogeneity is verified by SDS PAGE.
Form/Format
Supplied as a frozen liquid in 0.01M Tris, 0.5M sodium chloride, 0.02% sodium azide, 50% glycerol.
Applicable Applications for Jo-1, Thymus enzyme
ELISA (EL/EIA)
Application Notes
Recommended for use in standard solid phase immunoassays.
Unit Definition
One unit of activity is the amount of antigen that will generate an absorbance of 1.2 when assayed using standard procedure for enzyme immunoassay. Activity will vary with assay conditions/protocols.
Preparation and Storage
Product unstable above 0 degree C, aliquot and store frozen at -70 degree C. Avoid repeated freeze-thaw.
Related Product Information for Jo-1, Thymus enzyme
The Jo-1 antigen is histidyl-transfer ribonucleic acid (t-RNA) synthetase. This enzyme is partially responsible for attaching t-RNA to their cognate rRNA (2). The Jo-1 antigen migrates as a 53 kD protein on SDS-PAGE.
Anti-Jo-1 autoantibodies were originally described as precipitating autoantibodies in sera of patients with polymyositis. It was later realized that the anti-Jo-1 antibodies were specific for patients with polymyositis. The target for the anti-Jo-1 antibodies was one of a family of distinct cellular enzymes, the aminoacyl t-RNA synthetases (1).
The presence of autoantibodies against the Jo-1 antigen has been reported in up to 23% of polymyositis patients by immunodiffusion (3). Anti-Jo-1 antibodies are almost completely specific for myositis, being more common in polymyositis than dermato-myositis(4), and rare in children (5). The presence of anti-Jo-1 antibodies defines a distinct group of polymyositis patients with interstitial disease, arthritis, and fevers (6). The anti-Jo-1 response appears to be self-antigen driven, having a broad spectrotype over time undergoing isotype switching. Anti-Jo-1 antibodies also inhibit the function of human histidyl tRNA synthetase more than they do from other species (6).
Recent developments in ELISA meology have increased the sensitivity therefore the ability to quantitate patient antibody titers.
Anti-Jo-1 autoantibodies were originally described as precipitating autoantibodies in sera of patients with polymyositis. It was later realized that the anti-Jo-1 antibodies were specific for patients with polymyositis. The target for the anti-Jo-1 antibodies was one of a family of distinct cellular enzymes, the aminoacyl t-RNA synthetases (1).
The presence of autoantibodies against the Jo-1 antigen has been reported in up to 23% of polymyositis patients by immunodiffusion (3). Anti-Jo-1 antibodies are almost completely specific for myositis, being more common in polymyositis than dermato-myositis(4), and rare in children (5). The presence of anti-Jo-1 antibodies defines a distinct group of polymyositis patients with interstitial disease, arthritis, and fevers (6). The anti-Jo-1 response appears to be self-antigen driven, having a broad spectrotype over time undergoing isotype switching. Anti-Jo-1 antibodies also inhibit the function of human histidyl tRNA synthetase more than they do from other species (6).
Recent developments in ELISA meology have increased the sensitivity therefore the ability to quantitate patient antibody titers.
Product Categories/Family for Jo-1, Thymus enzyme
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Product Notes
The Jo-1, Thymus (Catalog #AAA653990) is an Enzyme produced from Calf Thymus and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Jo-1, Thymus can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA). Recommended for use in standard solid phase immunoassays. Researchers should empirically determine the suitability of the Jo-1, Thymus for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Jo-1, Thymus, Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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