CADM4 recombinant protein

CADM4 Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8121998

• Gene Names: CADM4; NECL4; TSLL2; IGSF4C; Necl-4; synCAM4
• Purity: >95% as determined by SDS-PAGE
• Synonyms: CADM4; CADM4 Protein; Human; Recombinant (hFc Tag); Human CADM4/IGSF4C/NECL-4 Protein (Fc Tag); IGSF4C Protein; Necl-4 Protein; NECL4 Protein; synCAM4 Protein; TSLL2 Protein; cell adhesion molecule 4; CADM4 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Tyr323

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Pro 21
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human CADM4 (NP_660339.1)(Met1-Tyr323) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CADM4 recombinant protein

Background: Immunoglobulin superfamily member 4C (IGSF4C), also known as CADM4 or NECL-4, is an immunoglobulin (Ig) superfamily molecule showing significant homology with a lung tumor suppressor, TSLC1. CADM4/IGSF4C/NECL-4 protein is mainly expressed in the kidney, bladder, and prostate in addition to the brain. Experiments have reported the biological significance of CADM4/IGSF4C/NECL-4 in the urinary tissues. An immunohistochemical study reveals that CADM4 is expressed at the cell-cell attachment sites in the renal tubules, the transitional epithelia of the bladder, and the glandular epithelia of the prostate. IGSF4-immunoreactivity (IR) was observed diffusely in the telencephalic wall, whereas it became rather confined to the subplate, the cortical plate and the subventricular zone as the development proceeded. IGSF4-IR gradually decreased after birth and disappeared in adulthood. IGSF4 remained at low levels throughout embryonic stage, whereas it increased after birth. These spatiotemporal patterns of the expression suggest that IGSF4 plays crucial roles in the development of both telencephalon and cerebellum. CADM4/IGSF4C/NECL-4 is ectopically expressed in adult T-cell leukemia (ATL) cells, providing not only a test marker for ATL, but also a possible therapeutic target against its invasion. The distinct roles of CADM4/IGSF4C/NECL-4 in the oncogenesis of carcinomas and ATL could be due to tissue-specific differences in the downstream cascades, and is a novel concept with respect to cell adhesion in human oncogenesis.

References

Williams YN, et al. (2006) Cell adhesion and prostate tumor-suppressor activity of TSLL2/IGSF4C, an immunoglobulin superfamily molecule homologous to TSLC1/IGSF4. Oncogene. 25(10): 1446-53.Ohta Y, et al. (2005) Spatiotemporal patterns of expression of IGSF4 in developing mouse nervous system. Brain Res Dev Brain Res. 156(1): 23-31.Shingai T, et al. (2003) Implications of nectin-like molecule-2 /IGSF4 /RA175 /SgIGSF /TSLC1 /SynCAM1 in cell-cell adhesion and transmembrane protein localization in epithelial cells. J Biol Chem. 278(37): 35421-7.

NCBI and Uniprot Product Information

• NCBI GI #: 21686977
• NCBI GeneID: 199731
• NCBI Accession #: NP_660339.1
• NCBI GenBank Nucleotide #: NM_145296.1
• UniProt Accession #: Q8NFZ8; Q9Y4A4; B2R7L5
• Molecular Weight: 42,785 Da
• NCBI Official Full Name: cell adhesion molecule 4
• NCBI Official Synonym Full Names: cell adhesion molecule 4
• NCBI Official Symbol: CADM4
• NCBI Official Synonym Symbols: NECL4; TSLL2; IGSF4C; Necl-4; synCAM4
• NCBI Protein Information: cell adhesion molecule 4; TSLC1-like 2; nectin-like 4; TSLC1-like protein 2; nectin-like protein 4; immunoglobulin superfamily member 4C; immunoglobulin superfamily, member 4C
• UniProt Protein Name: Cell adhesion molecule 4
• Protein Family: Cell adhesion molecule
• UniProt Gene Name: CADM4
• UniProt Synonym Gene Names: IGSF4C; NECL4; TSLL2; IgSF4C; NECL-4
• UniProt Entry Name: CADM4_HUMAN

Uniprot Description

CADM4: Involved in the cell-cell adhesion. Has calcium- and magnesium-independent cell-cell adhesion activity. May have tumor- suppressor activity. Belongs to the nectin family.

Protein type: Cell adhesion; Tumor suppressor; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.31

Cellular Component: integral to membrane

Biological Process: cell adhesion