Carbonic Anhydrase VIII Recombinant Protein | CA8 recombinant protein

Recombinant Carbonic Anhydrase VIII (CA8)

Cat. Num. AAA2030126

• Gene Names: CA8; CALS; CARP; CAMRQ3; CA-VIII
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Carbonic Anhydrase VIII; Recombinant Carbonic Anhydrase VIII (CA8); CA8 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MADLSFIEDT VAFPEKEEDE EEEEEGVEWG YEEGVEWGLV FPDANGEYQS PINLNSREAR YDPSLLDVRL SPNYVVCRDC EVTNDGHTIQ VILKSKSVLS GGPLPQGHEF ELYEVRFHWG RENQRGSEHT VNFKAFPMEL HLIHWNSTLF GSIDEAVGKP HGIAIIALFV QIGKEHVGLK AVTEILQDIQ YKGKSKTIPC FNPNTLLPDP LLRDYWVYEG SLTIPPCSEG VTWILFRYPLTISQLQIEEF RRLRTHVKGA ELVEGCDGIL GDNFRPTQPL SDRVIRAAFQ
• Sequence Length: 290

• Applicable Applications for CA8 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 36.7KDa
• Accurate Molecular Mass (KD): 37kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag
• Organism Species: Homo sapiens (Human)
• Fragment: Met1~Gln290
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 80477937
• NCBI GeneID: 767
• Molecular Weight: 32,973 Da
• NCBI Official Full Name: Carbonic anhydrase VIII
• NCBI Official Synonym Full Names: carbonic anhydrase VIII
• NCBI Official Symbol: CA8
• NCBI Official Synonym Symbols: CALS; CARP; CAMRQ3; CA-VIII
• NCBI Protein Information: carbonic anhydrase-related protein
• UniProt Protein Name: Carbonic anhydrase-related protein
• Protein Family: Carbonic anhydrase-related protein
• UniProt Gene Name: CA8
• UniProt Synonym Gene Names: CALS; CARP; CA-VIII
• UniProt Entry Name: CAH8_HUMAN

NCBI Description

The protein encoded by this gene was initially named CA-related protein because of sequence similarity to other known carbonic anhydrase genes. However, the gene product lacks carbonic anhydrase activity (i.e., the reversible hydration of carbon dioxide). The gene product continues to carry a carbonic anhydrase designation based on clear sequence identity to other members of the carbonic anhydrase gene family. The absence of CA8 gene transcription in the cerebellum of the lurcher mutant in mice with a neurologic defect suggests an important role for this acatalytic form. [provided by RefSeq, Jul 2008]

Uniprot Description

CA8: Does not have a carbonic anhydrase catalytic activity. Defects in CA8 are the cause of cerebellar ataxia mental retardation and dysequilibrium syndrome type 3 (CMARQ3). CMARQ3 is a congenital cerebellar ataxia associated with dysarthia, quadrupedal gait and mild mental retardation. Belongs to the alpha-carbonic anhydrase family.

Protein type: Lyase; Energy Metabolism - nitrogen

Chromosomal Location of Human Ortholog: 8q12.1

Cellular Component: cytoplasm

Molecular Function: carbonate dehydratase activity; zinc ion binding

Biological Process: phosphoinositide-mediated signaling; one-carbon compound metabolic process

Disease: Cerebellar Ataxia, Mental Retardation, And Dysequilibrium Syndrome 3