Human complement component 4 binding protein, alpha ELISA Kit | C4BPA elisa kit

Human C4 binding protein, C4BP ELISA Kit

Cat. Num. AAA700333

• Gene Names: C4BPA; PRP; C4BP
• Reactivity: Human
• Synonyms: complement component 4 binding protein; alpha; Human C4 binding protein; C4BP ELISA Kit; RP11-164O23.4; C4BP; PRP; C4b binding protein; alpha chain; complement component 4-binding protein; proline-rich protein; C4BPA elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human C4BPA. No significant cross-reactivity or interference between Human C4BPA and analogues was observed.
• Sequence Length: 597

• Samples: Serum, plasma and tissue homogenates
• Detection Range: 0.625 ng/ml-40 ng/ml
• Sensitivity: 0.156 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Complement
• Protein Biological Process 3: Complement pathway
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for C4BPA elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to C4BP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for C4BP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain C4BP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of C4BP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4502503
• NCBI GeneID: 722
• NCBI Accession #: NP_000706.1
• NCBI GenBank Nucleotide #: NM_000715.3
• UniProt Accession #: P04003; Q5VVQ8
• Molecular Weight: 67,033 Da
• NCBI Official Full Name: C4b-binding protein alpha chain
• NCBI Official Synonym Full Names: complement component 4 binding protein, alpha
• NCBI Official Symbol: C4BPA
• NCBI Official Synonym Symbols: PRP; C4BP
• NCBI Protein Information: C4b-binding protein alpha chain; proline-rich protein
• UniProt Protein Name: C4b-binding protein alpha chain
• Protein Family: C4b-binding protein
• UniProt Gene Name: C4BPA
• UniProt Synonym Gene Names: C4BP; C4bp; PRP
• UniProt Entry Name: C4BPA_HUMAN

NCBI Description

This gene encodes a member of a superfamily of proteins composed predominantly of tandemly arrayed short consensus repeats of approximately 60 amino acids. Along with a single, unique beta-chain, seven identical alpha-chains encoded by this gene assemble into the predominant isoform of C4b-binding protein, a multimeric protein that controls activation of the complement cascade through the classical pathway. The genes encoding both alpha and beta chains are located adjacent to each other on human chromosome 1 in the regulator of complement activation gene cluster. Two pseudogenes of this gene are also found in the cluster. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. Alpha chain binds C4b. It interacts also with anticoagulant protein S and with serum amyloid P component.