Bovine Complement Component 3a Receptor 1 ELISA Kit | C3aR1 elisa kit
Bovine Complement Component 3a Receptor 1 ELISA Kit
Gene Names
C3AR1; AZ3B; C3AR; HNFAG09
Reactivity
Bovine
Synonyms
Complement Component 3a Receptor 1; Bovine Complement Component 3a Receptor 1 ELISA Kit; C3aR1 elisa kit
Reactivity
Bovine
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ug/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Related Product Information for C3aR1 elisa kit
Intended Uses: This C3aR1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine C3aR1. This ELISA kit for research use only!
Principle of the Assay: C3aR1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-C3aR1 antibody and an C3aR1-HRP conjugate. The assay sample and buffer are incubated together with C3aR1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C3aR1 concentration since C3aR1 from samples and C3aR1-HRP conjugate compete for the anti-C3aR1 antibody binding site. Since the number of sites is limited, as more sites are occupied by C3aR1 from the sample, fewer sites are left to bind C3aR1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C3aR1 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: C3aR1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-C3aR1 antibody and an C3aR1-HRP conjugate. The assay sample and buffer are incubated together with C3aR1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C3aR1 concentration since C3aR1 from samples and C3aR1-HRP conjugate compete for the anti-C3aR1 antibody binding site. Since the number of sites is limited, as more sites are occupied by C3aR1 from the sample, fewer sites are left to bind C3aR1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C3aR1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for C3aR1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53,864 Da
NCBI Official Full Name
C3a anaphylatoxin chemotactic receptor
NCBI Official Synonym Full Names
complement component 3a receptor 1
NCBI Official Symbol
C3AR1
NCBI Official Synonym Symbols
AZ3B; C3AR; HNFAG09
NCBI Protein Information
C3a anaphylatoxin chemotactic receptor; C3a-R; complement component 3 receptor 1
UniProt Protein Name
C3a anaphylatoxin chemotactic receptor
Protein Family
UniProt Gene Name
C3AR1
UniProt Synonym Gene Names
AZ3B; C3R1; HNFAG09; C3AR; C3a-R
UniProt Entry Name
C3AR_HUMAN
Uniprot Description
C3aR: Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production. Belongs to the G-protein coupled receptor 1 family.
Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1
Chromosomal Location of Human Ortholog: 12p13.31
Cellular Component: integral to plasma membrane; plasma membrane
Molecular Function: G-protein coupled receptor activity; C3a anaphylatoxin receptor activity; complement component C3a receptor activity; phosphoinositide phospholipase C activity
Biological Process: G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; positive regulation of angiogenesis; metabolic process; blood circulation; complement receptor mediated signaling pathway; inflammatory response; chemotaxis
Research Articles on C3aR1
Similar Products
Product Notes
The Bovine C3aR1 c3ar1 (Catalog #AAA750307) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA750307 ELISA Kit recognizes Bovine C3aR1. It is sometimes possible for the material contained within the vial of "Complement Component 3a Receptor 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
