Structural polyprotein Recombinant Protein | C recombinant protein
Recombinant Rubella virus Structural polyprotein
NCBI and Uniprot Product Information
Uniprot Description
Function: Capsid protein interacts with genomic RNA and assembles into icosaedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of E2 at the cell membrane, leading to budding and formation of mature virions. Phosphorylation negatively regulates RNA-binding activity, possibly delaying virion assembly during the viral replication phase. Capsid protein dimerizes and becomes disulfide-linked in the virion, but this interaction seems not to be important for its biological function. Modulates genomic RNA replication. Modulates subgenomic RNA synthesis by interacting with human C1QBP/SF2P32. Induces both perinuclear clustering of mitochondria and the formation of electron-dense intermitochondrial plaques, both hallmarks of rubella virus infected cells. Induces apoptosis when expressed in transfected cells
By similarity.E2 envelope glycoprotein is responsible for viral attachment to target host cell, by binding to the cell receptor. Its transport to the plasma membrane depends on interaction with E1 protein
By similarity.E1 envelope glycoprotein is a class II viral fusion protein. Fusion activity is inactive as long as E1 is bound to E2 in mature virion. After virus attachment to target cell and clathrin-mediated endocytosis, acidification of the endosome would induce dissociation of E1/E2 heterodimer and concomitant trimerization of the E1 subunits. This E1 homotrimer is fusion active, and promotes release of viral nucleocapsid in cytoplasm after endosome and viral membrane fusion. E1 cytoplasmic tail modulates virus release, and the tyrosines residues are critical for this function
By similarity.
Subunit structure: Capsid protein homooligomerizes. Forms dimer shortly after synthesis, this dimer become disulfide-linked in the virion. Interacts with human C1QBP. E1 and E2 envelope glycoproteins heterodimerize
By similarity.
Subcellular location: Capsid protein: Virion
By similarity. Host cytoplasm
By similarity. Host mitochondrion
By similarity. Note: The capsid protein is concentrated around Golgi region
By similarity.E1 envelope glycoprotein: Virion membrane; Single-pass type I membrane protein
By similarity. Host Golgi apparatus membrane; Single-pass type I membrane protein
By similarity. Note: E1 and E2 form heterodimer in the endoplasmic reticulum before they are transported to and retained in the Golgi complex, where virus assembly occurs. E1 possesses an endoplasmic reticulum retention signal, and unassembled E2 and E1 subunits are retained in the endoplasmic reticulum. Presumably, assembly of E2 and E1 would mask the signal, thereby allowing transport of the heterodimer to the Golgi complex
By similarity.E2 envelope glycoprotein: Virion membrane; Single-pass type I membrane protein
By similarity. Host Golgi apparatus membrane; Single-pass type I membrane protein
By similarity. Note: E1 and E2 form heterodimer in the endoplasmic reticulum before they are transported to and retained in the Golgi complex, where virus assembly occurs. E1 possesses an endoplasmic reticulum retention signal, and unassembled E2 and E1 subunits are retained in the endoplasmic reticulum. Presumably, assembly of E2 and E1 would mask the signal, thereby allowing transport of the heterodimer to the Golgi complex
By similarity.
Domain: The signal peptide at the C-terminus remains bound to the mature capsid protein. It may have a role as an anchor peptide, localizing the capsid assembly at the Golgi complex, where budding occurs
By similarity.
Post-translational modification: Specific enzymatic cleavages in vivo yield mature proteins. An internal signal peptide at the capsid C-terminus directs E2-E1 sequences of the polyprotein precursor to the endoplasmic reticulum. Signal peptidase cleaves the precursor between Capsid and E2, releasing the capsid in the cytoplasm and the E2 N-terminus in ER. Another signal peptide at E2 C-terminus directs E1 to the ER, with a similar mechanism
By similarity.Capsid is phosphorylated on Ser-46 by host. This phosphorylation negatively regulates capsid protein RNA-binding activity. Dephosphorylated by human PP1A
By similarity.
Miscellaneous: Structural polyprotein is translated from a subgenomic RNA synthesized during togaviruses replication.
Sequence caution: The sequence CAA33016.1 differs from that shown. Reason: Extreme variation in vaccine RA27/3 lot.
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Product Notes
The Structural polyprotein c (Catalog #AAA1138153) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 583-1028. Partial, provide the complete extracellular domain of the E1 envelope glycoprotein chain. The amino acid sequence is listed below: EEAFTYLCTA PGCATQAPVP VRLAGVRFES KIVDGGCFAP WDLEATGACI CEIPTDVSCE GLGAWVPTAP CARIWNGTQR ACTFWAVNAY SSGGYAQLAS YFNPGGSYYK QYHPTACEVE PAFGHSDAAC WGFPTDTVMS VFALASYVQH PHKTVRVKFH TETRTVWQLS VAGVSCNVTT EHPFCNTPHG QLEVQVPPDP GDLVEYIMNH TGNQQSRWGL GSPNCHGPDW ASPVCQRHSP DCSRLVGATP ERPRLRLVDA DDPLLRTAPG PGEVWVTPVI GSQARKCGLH IRAGPYGHAT VEMPEWIHAH TTSDPWHPPG PLGLKFKTVR PVALPRTLAP PRNVRVTGCY QCGTPALVEG LAPGGGNCHL TVNGEDLGAF PPGKFVTAAL LNTPPPYQVS CGGESDRASA RVIDPAAQSF TGVVYGTHTT AVSETRQTWA EWAAAH . It is sometimes possible for the material contained within the vial of "Structural polyprotein, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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