c-erbB3, Fc Chimera Recombinant Protein
c-erbB3, Fc Chimera, Recombinant, Mouse (HER 3, ErbB3)
Reactivity
Mouse
Applications
Western Blot
Purity
Highly Purified90%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
Synonyms
c-erbB3; Fc Chimera; Recombinant; Mouse (HER 3; ErbB3); Fc Chimera recombinant protein
Reactivity
Mouse
Purity/Purification
Highly Purified
90%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
90%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
Form/Format
Supplied as a lyophilized powder in PBS. Reconstitute with sterile PBS to 0.1mg/ml.
Applicable Applications for c-erbB3, Fc Chimera recombinant protein
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 95.6kD protein migrates as an ~ 120-135kD protein in SDS-PAGE under reducing conditions.
Dilution: Western Blot: 95.6kD protein migrates as an ~ 120-135kD protein in SDS-PAGE under reducing conditions.
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute with sterile PBS and store at -20 degree C. Reconstituted product is stable for 3 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for c-erbB3, Fc Chimera recombinant protein
ErbB3, also called Her3 (human epidermal growth factor receptor 3) in humans, is a type I transmembrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors named for a viral oncogene.1-3 ErbB family members serve as receptors for the EGF family of growth factors.1-3 Mouse ErbB3 contains a 19 amino acid (aa) signal sequence, a 622 aa extracellular domain (ECD), a 24 aa transmembrane region, and a 677 aa cytoplasmic domain.4 Human ErbB3 has four isoforms created by intron read-through and truncation of the molecule.5 Three of these are secreted and at least one can inhibit ErbB3 activity.6 Little information is available concerning mouse ErbB3 isoforms. The mouse ErbB3 ECD shares 97%, 93%, 92%, 91%, 89% and 88% aa identity with rat, human, cow, horse, dog and opossum ErbB3, respectively. ErbB3 is found in epithelial cell layers of gastrointestinal, reproductive, urinary, endocrine and nervous systems, skin and muscle.3 Among ErbB family members, only ErbB3 lacks a working kinase domain, requiring heterodimerization with another ErbB receptor for signaling.1-3 The heterodimer of ErbB3 with ErbB2, which has no known ligands of its own, is expressed in the majority of breast, skin, ovary and gastrointestinal tumors and transduces a highly mitogenic signal in response to neuregulin 1 (NRG1; heuregulin 1) or NRG2.3, 7-9 These ligands also bind ErbB4.1 Signaling is aided by the six consensus binding motifs for the SH2 domain and one for the SH3 domain of the regulatory p85 subunit of phosphoinositide 3-kinase.10, 11 Deletion studies in mice demonstrate non-redundant roles for ErbB3 in development of Schwann cells, neural crest cells and heart valves.12, 13 ErbB3, ErbB2 and neuregulin are all required for formation of the sympathetic nervous system. (14)
Product Categories/Family for c-erbB3, Fc Chimera recombinant protein
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Product Notes
The c-erbB3, Fc Chimera (Catalog #AAA637727) is a Recombinant Protein and is intended for research purposes only. The product is available for immediate purchase. The c-erbB3, Fc Chimera, Recombinant, Mouse (HER 3, ErbB3) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's c-erbB3, Fc Chimera can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 95.6kD protein migrates as an ~ 120-135kD protein in SDS-PAGE under reducing conditions. Researchers should empirically determine the suitability of the c-erbB3, Fc Chimera for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "c-erbB3, Fc Chimera, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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