B And T-Lymphocyte Attenuator Recombinant Protein | BTLA recombinant protein

Recombinant B And T-Lymphocyte Attenuator (BTLA)

Cat. Num. AAA2033544

• Gene Names: BTLA; BTLA1; CD272
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 90%
• Synonyms: B And T-Lymphocyte Attenuator; Recombinant B And T-Lymphocyte Attenuator (BTLA); CD272; BTLA1; B-and T-lymphocyte-associated protein; BTLA recombinant protein

• Host: E Coli
• Purity/Purification: > 90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: E KATKRNDEEC PVQLTITRNSKQSARTGELF KIQCPVKYCV HRPNVTWCKH NGTICVPLEV SPQLYTSWEENQSVPVFVLH FKPIHLSDNG SYSCSTNFNS QVINSHSVTI HVRERTQNSSEHPLITVSDI PDATNASGPS TMEERPGRTW LLY
• Sequence Length: 289

• Applicable Applications for BTLA recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 21.2kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Glu30~Tyr183
• Organism Species: Mouse
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 145580621
• NCBI GeneID: 151888
• NCBI Accession #: NP_861445.3
• NCBI GenBank Nucleotide #: NM_181780.3
• Molecular Weight: 27,317 Da
• NCBI Official Full Name: B- and T-lymphocyte attenuator isoform 1
• NCBI Official Synonym Full Names: B and T lymphocyte associated
• NCBI Official Symbol: BTLA
• NCBI Official Synonym Symbols: BTLA1; CD272
• NCBI Protein Information: B- and T-lymphocyte attenuator
• UniProt Protein Name: B- and T-lymphocyte attenuator
• Protein Family: B- and T-lymphocyte attenuator
• UniProt Gene Name: BTLA
• UniProt Entry Name: BTLA_HUMAN

NCBI Description

This gene encodes a member of the immunoglobulin superfamily. The encoded protein contains a single immunoglobulin (Ig) domain and is a receptor that relays inhibitory signals to suppress the immune response. Alternative splicing results in multiple transcript variants. Polymorphisms in this gene have been associated with an increased risk of rheumatoid arthritis. [provided by RefSeq, Aug 2011]

Uniprot Description

BTLA: Lymphocyte inhibitory receptor which inhibits lymphocytes during immune response. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q13.2

Cellular Component: external side of plasma membrane; integral to plasma membrane; plasma membrane

Molecular Function: receptor activity

Biological Process: adaptive immune response; immune response-regulating cell surface receptor signaling pathway; negative regulation of alpha-beta T cell proliferation; negative regulation of B cell proliferation; T cell costimulation