Bruton Tyrosine Kinase / BTK Kinase Recombinant Protein | BTK recombinant protein

Recombinant Human Bruton Tyrosine Kinase / BTK Kinase Protein (His tag)

Cat. Num. AAA2545107

• Gene Names: BTK; AT; ATK; BPK; XLA; IMD1; AGMX1; PSCTK1
• Purity: > 85 % as determined by SDS-PAGE
• Synonyms: Bruton Tyrosine Kinase / BTK Kinase; Recombinant Human Bruton Tyrosine Kinase / BTK Kinase Protein (His tag); BTK; AGMX1; ATK; BPK; BTK recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 85 % as determined by SDS-PAGE
• Form/Format: Supplied as sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.0
• Sequence Length: 659

• Application Notes: The secreted recombinant human BTK consists of 670 amino acids and predicts a molecular mass of 77.8 KDa.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for BTK recombinant protein

Background: Bruton's tyrosine kinase (or BTK) is a type of kinase protein expressed in B lymphocytes and T cells. BTK contains a PH domain which binds phosphatidylinositol (3, 4, 5)-trisphosphate (PIP3). After binding to PIP3, BTK is induced to phosphorylate phospholipase C, which in turn hydrolyzes PIP2 into two second messagers, IP3 and DAG, which then modulate the activity of downstream proteins during B-cell signaling. Btk is also found implicated in the primary immunodeficiency disease X-linked agammaglobulinemia (Bruton's agammaglobulinemia). BTK played a key role in B-cell maturation as well as mast cell activation through the high-affinity IgE receptor. Patients with X-linked agammaglobulinemia have normal pre-B cell populations in their bone marrow but these B-cells can not mature and enter the circulation.

Description: A DNA sequence encoding the human BTK (NP_000052.1) (Met 1-Ser 659) was expressed with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 4557377
• NCBI GeneID: 695
• NCBI Accession #: NP_000052.1
• NCBI GenBank Nucleotide #: NM_000061.2
• UniProt Accession #: Q06187; Q32ML5; B2RAW1
• Molecular Weight: 79,937 Da
• NCBI Official Full Name: tyrosine-protein kinase BTK isoform 1
• NCBI Official Synonym Full Names: Bruton tyrosine kinase
• NCBI Official Symbol: BTK
• NCBI Official Synonym Symbols: AT; ATK; BPK; XLA; IMD1; AGMX1; PSCTK1
• NCBI Protein Information: tyrosine-protein kinase BTK
• UniProt Protein Name: Tyrosine-protein kinase BTK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: BTK
• UniProt Synonym Gene Names: AGMX1; ATK; BPK; ATK; BPK

NCBI Description

The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2013]

Uniprot Description

Btk: a tyrosine kinase of the Tec family. Plays a crucial role in B-cell ontogeny. Defects cause X-linked agammaglobulinemia, an immunodeficiency characterized by failure to produce mature B lymphocyte cells and associated with a failure of Ig heavy chain rearrangement. Truncated splice forms found in childhood leukemias may underlie radiation resistance of tumors through inhibition of apoptosis.

Protein type: EC 2.7.10.2; Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); TK group; Tec family

Chromosomal Location of Human Ortholog: Xq22.1

Cellular Component: cytoplasm; cytosol; extrinsic to internal side of plasma membrane; intracellular membrane-bound organelle; lipid raft; nucleus; plasma membrane

Molecular Function: ATP binding; identical protein binding; non-membrane spanning protein tyrosine kinase activity; phosphatidylinositol-3,4,5-triphosphate binding; protein binding; protein-tyrosine kinase activity; receptor binding

Biological Process: activation of NF-kappaB transcription factor; adaptive immune response; B cell activation; B cell receptor signaling pathway; calcium-mediated signaling; innate immune response; mesoderm development; MyD88-dependent toll-like receptor signaling pathway; peptidyl-tyrosine phosphorylation; positive regulation of B cell differentiation; protein amino acid phosphorylation; regulation of B cell apoptosis; regulation of B cell cytokine production; regulation of cell proliferation; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Agammaglobulinemia, X-linked; Isolated Growth Hormone Deficiency, Type Iii