Hepatocyte Growth Factor Active Protein
Hepatocyte Growth Factor, Recombinant, Human (HGF, Scatter Factor, SF, Hepatopoietin A)
Purity
Highly Purified98% by SDS-PAGE and HPLC analyses. Endotoxin: 0.1ng/ug (1EU/ug)
Synonyms
Hepatocyte Growth Factor; Recombinant; Human (HGF; Scatter Factor; SF; Hepatopoietin A); Hepatocyte Growth Factor active protein
Host
BTI-Tn-5B1-4 High-5 insect cells.
Purity/Purification
Highly Purified
98% by SDS-PAGE and HPLC analyses. Endotoxin: 0.1ng/ug (1EU/ug)
98% by SDS-PAGE and HPLC analyses. Endotoxin: 0.1ng/ug (1EU/ug)
Form/Format
Supplied as a lyophilized powder from 0.1M L-arginine HCl, 10mM Tris, 200mM sodium chloride, pH 8.0. Reconstitute with sterile ddH2O and a carrier protein to 0.5mg/ml. Do not vortex
Sequence
alph achain: QRKRRNTIHE FKKSAKTTLI KIDPALKIKT KKVNTADQCA NRCTRNKGLP FTCKAFVFDK ARKQCLWFPF NSMSSGVKKE FGHEFDLYEN KDYIRNCIIG KGRSYKGTVS ITKSGIKCQP WSSMIPHEHS FLPSSYRGKD LQENYCRNPR GEEGGPWCFT SNPEVRYEVC DIPQCSEVEC MTCNGESYRG LMDHTESGKI CQRWDHQTPH RHKFLPERYP DKGFDDNYCR NPDGQPRPWC YTLDPHTRWE YCAIKTCADN TMNDTDVPLE TTECIQGQGE GYRGTVNTIW NGIPCQRWDS QYPHEHDMTP ENFKCKDLRE NYCRNPDGSE SPWCFTTDPN IRVGYCSQIP NCDMSHGQDC YRGNGKNYMG NLSQTRSGLT CSMWDKNMED LHRHIFWEPD ASKLNENYCR NPDDDAHGPW CYTGNPLIPW DYCPISRCEG DTTPTIVNLD HPVISCAKTK QLR
Biological Activity
The ED50 was determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells was found to be in the range of 20-40ng/ml.
Country of Origin
USA
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Reconstitute by adding sterile ddH2O and a carrier protein. Aliquot and store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C with a carrier protein. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Hepatocyte Growth Factor active protein
HGF is a mesenchymally derived potent mitogen for mature parenchymal hepatocyte cells and acts as a growth factor for a broad spectrum of tissues and cell types. HGF signals through a transmembrane tyrosine kinase receptor known as MET. Activities of HGF include induction of cell proliferation, motility, morphogenesis, inhibition of cell growth, and enhancement of neuron survival. HGF is a crucial mitogen for liver regeneration processes, especially after partial hepatectomy and other liver injuries. Human and murine HGF are cross-reactive. Human HGF is expressed as a linear 697 amino acid polypeptide precursor glycoprotein. Proteolytic processing of this precursor generates the biologically active form of HGF, which consists of two polypeptide chains (alpha-chain and beta-chain) held by a single disulfide bond resulting in formation of a biologically active heterodimer. The alpha-chain consists of 463 amino acid residues and four kringle domains. The beta-chain consists of 234 amino acid residues.
Product Categories/Family for Hepatocyte Growth Factor active protein
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
80kD
NCBI Official Full Name
hepatocyte growth factor
Protein Family
Similar Products
Product Notes
The Hepatocyte Growth Factor (Catalog #AAA650084) is an Active Protein produced from BTI-Tn-5B1-4 High-5 insect cells. and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: alph achain: QRKRRNTIHE FKKSAKTTLI KIDPALKIKT KKVNTADQCA NRCTRNKGLP FTCKAFVFDK ARKQCLWFPF NSMSSGVKKE FGHEFDLYEN KDYIRNCIIG KGRSYKGTVS ITKSGIKCQP WSSMIPHEHS FLPSSYRGKD LQENYCRNPR GEEGGPWCFT SNPEVRYEVC DIPQCSEVEC MTCNGESYRG LMDHTESGKI CQRWDHQTPH RHKFLPERYP DKGFDDNYCR NPDGQPRPWC YTLDPHTRWE YCAIKTCADN TMNDTDVPLE TTECIQGQGE GYRGTVNTIW NGIPCQRWDS QYPHEHDMTP ENFKCKDLRE NYCRNPDGSE SPWCFTTDPN IRVGYCSQIP NCDMSHGQDC YRGNGKNYMG NLSQTRSGLT CSMWDKNMED LHRHIFWEPD ASKLNENYCR NPDDDAHGPW CYTGNPLIPW DYCPISRCEG DTTPTIVNLD HPVISCAKTK QLR. It is sometimes possible for the material contained within the vial of "Hepatocyte Growth Factor, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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