BTD recombinant protein

BTD, 44-545aa, Human, His-tag, Baculovirus

Cat. Num. AAA206468

• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: BTD; 44-545aa; Human; His-tag; Baculovirus; Biotinidase isoform 1; biotinidase; BTD recombinant protein

• Host: Baculovirus
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: AHTGEESVAD HHEAEYYVAA VYEHPSILSL NPLALISRQE ALELMNQNLD IYEQQVMTAA QKDVQIIVFP EDGIHGFNFT RTSIYPFLDF MPSPQVVRWN PCLEPHRFND TEVLQRLSCM AIRGDMFLVA NLGTKEPCHS SDPRCPKDGR YQFNTNVVFS NNGTLVDRYR KHNLYFEAAF DVPLKVDLIT FDTPFAGRFG IFTCFDILFF DPAIRVLRDY KVKHVVYPTA WMNQLPLLAA IEIQKAFAVA FGINVLAANV HHPVLGMTGS GIHTPLESFW YHDMENPKSH LIIAQVAKNP VGLIGAENAT GETDPSHSKF LKILSGDPYC EKDAQEVHCD EATKWNVNAP PTFHSEMMYD NFTLVPVWGK EGYLHVCSNG LCCYLLYERP TLSKELYALG VFDGLHTVHG TYYIQVCALV RCGGLGFDTC GQEITEATGI FEFHLWGNFS TSYIFPLFLT SGMTLEVPDQ LGWENDHYFL RKSRLSSGLV TAALYGRLYE RDLEHHHHHH
• Sequence Length: 545

• Applicable Applications for BTD recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for BTD recombinant protein

BTD, also known as biotinidase isoform 1, is a member of the nitrilase superfamily, which consists of 12 families of nitrilases, amidases, carbamylases, and N-acyltrasferases. It catalyzes the hydrolysis of biocytin, the product of biotin-dependent carboxylase degradation, to biotin and lysine. It may have an important regulatory role in chromatin/DNA function. Recombinant human BTD, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for BTD recombinant protein

Signal Transduction; Metabolism

References

Hymes J., et al. (2001) Hum Mutat. 18:375-381.; Cole H., et al. (1994) J Biol Chem. 269:6566-6570.

NCBI and Uniprot Product Information

• NCBI GI #: 528524481
• NCBI GeneID: 686
• NCBI Accession #: NP_001268652
• NCBI GenBank Nucleotide #: NM_001281723.2
• UniProt Accession #: P43251; Q96EM9; A6NHF2; B2R865; B4DFX1; B4DLJ9; B7Z7C9; F8W1Q3
• Molecular Weight: 57.8kDa (510aa) 50-70kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: biotinidase isoform 1
• NCBI Official Synonym Full Names: biotinidase
• NCBI Official Symbol: BTD
• NCBI Protein Information: biotinidase
• UniProt Protein Name: Biotinidase
• Protein Family: Biotinidase
• UniProt Gene Name: BTD
• UniProt Synonym Gene Names: Biotinase

NCBI Description

The protein encoded by this gene functions to recycle protein-bound biotin by cleaving biocytin (biotin-epsilon-lysine), a normal product of carboxylase degradation, resulting in regeneration of free biotin. The encoded protein has also been shown to have biotinyl transferase activity. Mutations in this gene are associated with biotinidase deficiency. Multiple transcript variants encoding different isoforms have been described. [provided by RefSeq, Aug 2013]

Uniprot Description

Catalytic release of biotin from biocytin, the product of biotin-dependent carboxylases degradation.