Mouse Betacellulin ELISA Kit | bTC elisa kit

Mouse Betacellulin ELISA Kit

Cat. Num. AAA728526

• Reactivity: Mouse
• Synonyms: Betacellulin; Mouse Betacellulin ELISA Kit; bTC elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This betaTC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Moue betaTC. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for bTC elisa kit

Principle of the Assay: betaTC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-betaTC antibody and an betaTC-HRP conjugate. The assay sample and buffer are incubated together with betaTC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the betaTC concentration since betaTC from samples and betaTC-HRP conjugate compete for the anti-betaTC antibody binding site. Since the number of sites is limited, as more sites are occupied by betaTC from the sample, fewer sites are left to bind betaTC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The betaTC concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 265786
• NCBI GeneID: 685
• Molecular Weight: 19,746 Da
• NCBI Official Full Name: betacellulin
• NCBI Official Synonym Full Names: betacellulin
• NCBI Official Symbol: BTC
• NCBI Protein Information: probetacellulin
• UniProt Protein Name: Probetacellulin
• Protein Family: Probetacellulin
• UniProt Gene Name: BTC
• UniProt Synonym Gene Names: BTC
• UniProt Entry Name: BTC_HUMAN

NCBI Description

The protein encoded by this gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. [provided by RefSeq, Jul 2008]

Uniprot Description

BTC: Growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.

Protein type: Membrane protein, integral; Ligand, receptor tyrosine kinase; Cell cycle regulation

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular space; extracellular region; plasma membrane; integral to membrane

Molecular Function: protein binding; growth factor activity; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of fibroblast proliferation; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; positive regulation of mitosis; positive regulation of cell proliferation; innate immune response; positive regulation of cell differentiation; negative regulation of apoptosis