BRD2 bromodomain Recombinant Protein | BRD2 recombinant protein

BRD2 bromodomain (1-455 aa) (His-Tag), human recombinant

Cat. Num. AAA845316

• Gene Names: BRD2; FSH; NAT; RNF3; FSRG1; RING3; D6S113E
• Applications: SDS-Page
• Purity: >90% by SDS-PAGE
• Synonyms: BRD2 bromodomain; BRD2 bromodomain (1-455 aa) (His-Tag); human recombinant; RING3; RNF3; Bromodomain containing 4; D6S113E; FSH; FSRG1; NAT; BRD2 recombinant protein

• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Formulation: 1 mg/ml in Phosphate buffer saline (pH 7.4) containing 10% glycerol and 1 mM DTT.; Form: Liquid
• Sequence Positions: 1-455
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMLQNVTP HNKLPGEGNA GLLGLGPEAA APGKRIRKPS LLYEGFESPT MASVPALQLT PANPPPPEVS NPKKPGRVTN QLQYLHKVVM KALWKHQFAW PFRQPVDAVK LGLPDYHKII KQPMDMGTIK RRLENNYYWA ASECMQDFNT MFTNCYIYNK PTDDIVLMAQ TLEKIFLQKV ASMPQEEQEL VVTIPKNSHK KGAKLAALQG SVTSAHQVPA VSSVSHTALY TPPPEIPTTV LNIPHPSVIS SPLLKSLHSA GPPLLAVTAA PPAQPLAKKK GVKRKADTTT PTPTAILAPG SPASPPGSLE PKAARLPPMR RESGRPIKPP RKDLPDSQQQ HQSSKKGKLS EQLKHCNGIL KELLSKKHAA YAWPFYKPVD ASALGLHDYH DIIKHPMDLS TVKRKMENRD YRDAQEFAAD VRLMFSNCYK YNPPDHDVVA MARKLQDVFE FRYAKMPD
• Sequence Length: 801

• Applicable Applications for BRD2 recombinant protein: SDS-PAGE
• Source: E.coli
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Testing Data

Related Product Information for BRD2 recombinant protein

Background: The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as "readers" of histone acetylation marks regulating the transcription of target promoters. The BET family of proteins, defined by tandem Bromodomains and an Extra Terminal domain, include BRD2, BRD3, BRD4, and BRDT. The BET proteins play a key role in many cellular processes, including inflammatory gene expression, mitosis, and viral/host interactions. The isolated individual or tandem bromodomains of BRD2 and BRD4 have been shown to bind acetylated histone tails, serving to couple histone acetylation marks to the transcriptional regulation of target promoters. Small molecule inhibitors of these interactions hold promise as useful therapeutics for human disease. This protein can be used for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.

Product Categories/Family for BRD2 recombinant protein

Epigenetics; Reader Domains; Bromodomains; BromodomainsProteins and Enzymes; Other Proteins & EnzymesProteins and Enzymes; Animal-Free Origin (AOF) Recombinant Proteins & EnzymesProteins and Enzymes; Proteins and Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 164419758
• NCBI GeneID: 6046
• NCBI Accession #: NP_001106653.1
• NCBI GenBank Nucleotide #: NM_001113182.2
• UniProt Accession #: P25440; O00699; O00700; Q15310; Q5STC9; Q63HQ9; Q658Y7; Q6P3U2; Q969U4; A2AAU0; B0S7P0; B1AZT1
• Molecular Weight: 52.8 kDa (478 aa, 1-455 aa + His Tag), confirmed by MALDI-TOF.
• NCBI Official Full Name: bromodomain-containing protein 2 isoform 1
• NCBI Official Synonym Full Names: bromodomain containing 2
• NCBI Official Symbol: BRD2
• NCBI Official Synonym Symbols: FSH; NAT; RNF3; FSRG1; RING3; D6S113E
• NCBI Protein Information: bromodomain-containing protein 2
• UniProt Protein Name: Bromodomain-containing protein 2
• Protein Family: Bromodomain-containing protein
• UniProt Gene Name: BRD2
• UniProt Synonym Gene Names: KIAA9001; RING3
• UniProt Entry Name: BRD2_HUMAN

NCBI Description

This gene encodes a transcriptional regulator that belongs to the BET (bromodomains and extra terminal domain) family of proteins. This protein associates with transcription complexes and with acetylated chromatin during mitosis, and it selectively binds to the acetylated lysine-12 residue of histone H4 via its two bromodomains. The gene maps to the major histocompatability complex (MHC) class II region on chromosome 6p21.3, but sequence comparison suggests that the protein is not involved in the immune response. This gene has been implicated in juvenile myoclonic epilepsy, a common form of epilepsy that becomes apparent in adolescence. Multiple alternatively spliced variants have been described for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

BRD2: an atypical protein kinase with two bromodomains that bind to acetylated lysine residues. Specifically interacts with transcriptionally active chromatin. Elevated protein kinase activity in leukemias. Transgenic mice overexpressing BRD2 in the lymphoid system develop diffuse large-cell lymphoma. Interacts with E2F1 and with histone H4 acetylated at Lys-13. May play a role in spermatogenesis or folliculogenesis. Genetic evidence links the BRD2 gene to both juvenile myoclonic epilepsy and photoparoxysomal responses. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, atypical; ATYPICAL group; BRD family

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; chromatin binding

Biological Process: regulation of transcription from RNA polymerase II promoter; nucleosome assembly; transcription, DNA-dependent; spermatogenesis; chromatin modification