BPGM recombinant protein

BPGM, 1-259aa, Human, His tag, E Coli

Cat. Num. AAA203620

• Gene Names: BPGM; DPGM
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: BPGM; 1-259aa; Human; His tag; E Coli; Bisphosphoglycerate mutase; BPG-dependent PGAM; Bisphosphoglycerate mutase 2; 3-bisphosphoglycerate mutase; erythrocyte; 2; 3-bisphosphoglycerate synthase; Ab2 098; AI323730 AL022789; BPG dependent PGAM; C86192.; BPGM recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 10% glycerol
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MSKYKLIMLR HGEGAWNKEN RFCSWVDQKL NSEGMEEARN CGKQLKALNF EFDLVFTSVL NRSIHTAWLI LEELGQEWVP VESSWRLNER HYGALIGLNR EQMALNHGEE QVRLWRRSYN VTPPPIEESH PYYQEIYNDR RYKVCDVPLD QLPRSESLKD VLERLLPYWN ERIAPEVLRG KTILISAHGN SSRALLKHLE GISDEDIINI TLPTGVPILL ELDENLRAVG PHQFLGDQEA IQAAIKKVED QGKVKQAKKL EHHHHHH
• Sequence Length: 259

• Applicable Applications for BPGM recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for BPGM recombinant protein

Bisphosphoglycerate mutase (BPGM) is an enzyme unique to erythrocytes and placental cells. This protein plays a major role in regulating hemoglobin oxygen affinity as a consequence of controlling 2,3-BPG concentration. It is responsible for the catalytic synthesis of 2,3-Bisphosphoglycerate (2,3-BPG) from 1,3-BPG. BPGM also has a mutase and a phosphatase function, but these are much less active. Recombinant human BPGM, fused to His-tag at C-terminus, was expressed in E Coli and purified by using conventional chromatography techniques

Product Categories/Family for BPGM recombinant protein

Enzymes & Proteases

References

Pritlove DC., et al. (2006) Placenta. 27 (8):924-7; Wang Y., et al. (2006) J. Biol. Chem. 281 (51):39642-8.;

NCBI and Uniprot Product Information

• NCBI GI #: 4502445
• NCBI GeneID: 669
• NCBI Accession #: NP_001715
• NCBI GenBank Nucleotide #: NM_001724.4
• UniProt Accession #: P07738; A4D1N9
• Molecular Weight: 31kDa (267aa), confirmed by MALDI-TOF
• NCBI Official Full Name: bisphosphoglycerate mutase
• NCBI Official Synonym Full Names: bisphosphoglycerate mutase
• NCBI Official Symbol: BPGM
• NCBI Official Synonym Symbols: DPGM
• NCBI Protein Information: bisphosphoglycerate mutase
• UniProt Protein Name: Bisphosphoglycerate mutase
• Protein Family: Bisphosphoglycerate mutase
• UniProt Gene Name: BPGM
• UniProt Synonym Gene Names: DPGM

NCBI Description

2,3-diphosphoglycerate (2,3-DPG) is a small molecule found at high concentrations in red blood cells where it binds to and decreases the oxygen affinity of hemoglobin. This gene encodes a multifunctional enzyme that catalyzes 2,3-DPG synthesis via its synthetase activity, and 2,3-DPG degradation via its phosphatase activity. The enzyme also has phosphoglycerate phosphomutase activity. Deficiency of this enzyme increases the affinity of cells for oxygen. Mutations in this gene result in hemolytic anemia. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Sep 2009]

Uniprot Description

Plays a major role in regulating hemoglobin oxygen affinity by controlling the levels of its allosteric effector 2,3-bisphosphoglycerate (2,3-BPG). Also exhibits mutase (EC 5.4.2.1) activity.