Bovine Ubiquitin (Ub) ELISA Kit | Ub elisa kit

Bovine Ubiquitin (Ub) ELISA Kit

Cat. Num. AAA2020593

• Gene Names: UBC; HMG20
• Reactivity: Bovine
• Synonyms: Ubiquitin (Ub); Bovine Ubiquitin (Ub) ELISA Kit; Ub elisa kit

• Reactivity: Bovine
• Specificity: This assay has high sensitivity and excellent specificity for detection of Ubiquitin (Ub); No significant cross-reactivity or interference between Ubiquitin (Ub) and analogues was observed.
• Sequence Length: 685

• Samples: Serum, plasma and other biological fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL The standard curve concentrations used for the ELISA's were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL
• Sensitivity: Typically less than 0.059ng/mL.
• Organism Species: Bos taurus; Bovine (Cattle)
• ELISA Type: Sandwich
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ubiquitin (Ub) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ubiquitin (Ub) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Ub elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin (Ub). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquitin (Ub). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquitin (Ub), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ubiquitin (Ub) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 340068
• NCBI GeneID: 7316
• UniProt Accession #: P0CG48; P02248; P02249; P02250; P62988; Q29120; Q6LBL4; Q6LDU5; Q8WYN8; Q91887; Q91888; Q9BWD6
• Molecular Weight: 77,039 Da
• NCBI Official Full Name: ubiquitin
• NCBI Official Synonym Full Names: ubiquitin C
• NCBI Official Symbol: UBC
• NCBI Official Synonym Symbols: HMG20
• NCBI Protein Information: polyubiquitin-C
• UniProt Protein Name: Polyubiquitin-C
• UniProt Gene Name: UBC
• UniProt Entry Name: UBC_HUMAN

NCBI Description

This gene represents a ubiquitin gene, ubiquitin C. The encoded protein is a polyubiquitin precursor. Conjugation of ubiquitin monomers or polymers can lead to various effects within a cell, depending on the residues to which ubiquitin is conjugated. Ubiquitination has been associated with protein degradation, DNA repair, cell cycle regulation, kinase modification, endocytosis, and regulation of other cell signaling pathways. [provided by RefSeq, Aug 2010]

Uniprot Description

UBC: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Belongs to the ubiquitin family.

Protein type: Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 12q24.3

Cellular Component: nucleoplasm; plasma membrane; endosome membrane; cytosol

Molecular Function: protein binding; protease binding

Biological Process: circadian rhythm; I-kappaB kinase/NF-kappaB cascade; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; nerve growth factor receptor signaling pathway; viral reproduction; positive regulation of apoptosis; activation of MAPK activity; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; endosome transport; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; regulation of apoptosis; toll-like receptor 5 signaling pathway; antigen processing and presentation of peptide antigen via MHC class I; transforming growth factor beta receptor signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I; JNK cascade; G2/M transition of mitotic cell cycle; toll-like receptor 4 signaling pathway; regulation of interferon type I production; glycogen biosynthetic process; positive regulation of I-kappaB kinase/NF-kappaB cascade; fibroblast growth factor receptor signaling pathway; transcription, DNA-dependent; glucose metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; Notch receptor processing; virus assembly; toll-like receptor 2 signaling pathway; carbohydrate metabolic process; viral protein processing; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; negative regulation of interferon type I production; G1/S transition of mitotic cell cycle; negative regulation of apoptosis; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of epidermal growth factor receptor signaling pathway; apoptosis; pathogenesis; viral infectious cycle; negative regulation of transcription from RNA polymerase II promoter; toll-like receptor 10 signaling pathway; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; positive regulation of interferon type I production; transmembrane transport; epidermal growth factor receptor signaling pathway; transcription initiation from RNA polymerase II promoter; Notch signaling pathway; cytokine and chemokine mediated signaling pathway; MyD88-independent toll-like receptor signaling pathway; DNA repair; MyD88-dependent toll-like receptor signaling pathway; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; toll-like receptor signaling pathway; innate immune response; gene expression; mitotic cell cycle; negative regulation of transforming growth factor beta receptor signaling pathway