Typical Testing Data/Standard Curve (for reference only)
Bovine Obestatin ELISA Kit | Obestatin elisa kit
Bovine Obestatin ELISA Kit
Reactivity
Bovine
Synonyms
Obestatin; Bovine Obestatin ELISA Kit; Obestatin elisa kit
Reactivity
Bovine
Samples
Human Serum
Sensitivity
1.0 U/ml.
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Obestatin elisa kit
Intended Uses: This Nucleo-h IgG ELISA is a solid phase enzyme immunoassay with human native nucleosomes isolated from the eukaryotic cell line HeLa for the quantitative and qualitative detection of antibodies against nucleosomes and their components dsDNA and histones in human serum. For research use only.
Principle of the Assay: Serum samples diluted 1:101 are incubated in the microplates coated with the specific antigen. Antibodies, if present in the specimen, bind to the antigen. The unbound fraction is washed off in the following step. Afterwards anti-human immunoglobulins conjugated to horseradish peroxidase (conjugate) are incubated and react with the antigen-antibody complex of the samples in the microplates. Unbound conjugate is washed off in the following step. Addition of TMB-substrate generates an enzymatic colorimetric (blue) reaction, which is stopped by diluted acid (color changes to yellow). The intensity of color formation from the chromogen is a function of the amount of conjugate bound to the antigen-antibody complex and this is proportional to the initial concentration of the respective antibodies in the sample!!Background/Introduction: Nucleosomes are the basic structure of chromatin important for the compaction of DNA in the nucleus. A nucleosome comprises of four histones H2A, H2B, H3 and H4 forming an octamer and 146 bp of dsDNA wrapped 1 ¾ turns around the core (H2A, H2B, H3, H4)2 octamer. Histone H1 interacts with the nucleosome and together with linked-DNA connects neighboring nucleosomes.
Principle of the Assay: Serum samples diluted 1:101 are incubated in the microplates coated with the specific antigen. Antibodies, if present in the specimen, bind to the antigen. The unbound fraction is washed off in the following step. Afterwards anti-human immunoglobulins conjugated to horseradish peroxidase (conjugate) are incubated and react with the antigen-antibody complex of the samples in the microplates. Unbound conjugate is washed off in the following step. Addition of TMB-substrate generates an enzymatic colorimetric (blue) reaction, which is stopped by diluted acid (color changes to yellow). The intensity of color formation from the chromogen is a function of the amount of conjugate bound to the antigen-antibody complex and this is proportional to the initial concentration of the respective antibodies in the sample!!Background/Introduction: Nucleosomes are the basic structure of chromatin important for the compaction of DNA in the nucleus. A nucleosome comprises of four histones H2A, H2B, H3 and H4 forming an octamer and 146 bp of dsDNA wrapped 1 ¾ turns around the core (H2A, H2B, H3, H4)2 octamer. Histone H1 interacts with the nucleosome and together with linked-DNA connects neighboring nucleosomes.
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Product Notes
The Bovine Obestatin (Catalog #AAA755984) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA755984 ELISA Kit recognizes Bovine Obestatin. It is sometimes possible for the material contained within the vial of "Obestatin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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