Typical Testing Data/Standard Curve (for reference only)
Bovine Interferon-induced GTP-binding protein Mx1 (MX1) ELISA Kit | MX1 elisa kit
Bovine Interferon-induced GTP-binding protein Mx1 (MX1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: MX1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-MX1 antibody and an MX1-HRP conjugate. The assay sample and buffer are incubated together with MX1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MX1 concentration since MX1 from samples and MX1-HRP conjugate compete for the anti-MX1 antibody binding site. Since the number of sites is limited, as more sites are occupied by MX1 from the sample, fewer sites are left to bind MX1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MX1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a guanosine triphosphate (GTP)-metabolizing protein that participates in the cellular antiviral response. The encoded protein is induced by type I and type II interferons and antagonizes the replication process of several different RNA and DNA viruses. There is a related gene located adjacent to this gene on chromosome 21, and there are multiple pseudogenes located in a cluster on chromosome 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]
Uniprot Description
MX1: Dynamin-like GTPase acting as a cell-autonomous host restriction factor against many viral pathogens. Shows activity against influenza viruses, rhabdovirus VSV, bunyavirus LACV, Thogoto virus, measles virus, Hantaan virus, Coxsackie virus CVB, Rift Valley fever virus RVFV, hepatitis B virus HBV, and Crimean- Congo hemorrhagic fever virus CCHFV. Belongs to the dynamin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Hydrolase; Apoptosis
Chromosomal Location of Human Ortholog: 21q22.3
Cellular Component: endoplasmic reticulum membrane; nuclear membrane; perinuclear region of cytoplasm; cytoplasm; cytosol
Molecular Function: GTPase activity; protein binding; GTP binding
Biological Process: negative regulation of viral genome replication; apoptosis; metabolic process; cytokine and chemokine mediated signaling pathway; response to virus; innate immune response; defense response; signal transduction; defense response to virus
Research Articles on MX1
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Product Notes
The Bovine MX1 mx1 (Catalog #AAA7218016) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7218016 ELISA Kit recognizes Bovine MX1. It is sometimes possible for the material contained within the vial of "Interferon-induced GTP-binding protein Mx1 (MX1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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