Bovine Eukaryotic initiation factor 4A-I (EIF4A1) ELISA Kit | EIF4A1 elisa kit

Bovine Eukaryotic initiation factor 4A-I (EIF4A1) ELISA Kit

Cat. Num. AAA2800147

• Reactivity: Bovine
• Synonyms: Eukaryotic initiation factor 4A-I (EIF4A1); Bovine Eukaryotic initiation factor 4A-I (EIF4A1) ELISA Kit; DDX2A; EIF-4A; EIF4A; eukaryotic initiation factor 4AI; eukaryotic translation initiation factor 4A isoform 1; EIF4A1 elisa kit

• Reactivity: Bovine
• Specificity: This assay has high sensitivity and excellent specificity for detection of Bovine EIF4A1. No significant cross-reactivity or interference between Bovine EIF4A1 and analogues was observed.
• Sequence Length: 406

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for EIF4A1 elisa kit

Description: This assay employs a two-site sandwich ELISA to quantitate EIF4A1 in samples. An antibody specific for EIF4A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EIF4A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EIF4A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EIF4A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: The eukaryotic initiation factor-4A family consists of 2 closely related genes, EIF4A1 and EIF4A2. These factors are required for the binding of mRNA to 40S ribosomal subunits. Nielsen et al. (1985) cloned eif4a1 cDNAs from mouse liver. They identified 2 distinct cDNAs differing in their untranslated regions. The sizes of these cDNAs correspond to 2 discrete mRNA bands of 2.0 and 1.6 kb seen on Northern blots of both mouse and human cells. Nielsen and Trachsel (1988) found that eif4a1 was expressed at similar levels in all mouse tissues examined, while eif4a2 had a much more varied pattern of expression. The EIF4A1 cDNA encodes a predicted 406-amino acid polypeptide with 92.7% amino acid similarity to the mouse protein. Kukimoto et al. (1997) characterized the promoter region of human EIF4A1.

NCBI and Uniprot Product Information

• NCBI GI #: 77735407
• NCBI GeneID: 504958
• NCBI Accession #: NP_001029400.1
• NCBI GenBank Nucleotide #: NM_001034228.1
• UniProt Accession #: Q3SZ54
• Molecular Weight: 46,154 Da
• NCBI Official Full Name: eukaryotic initiation factor 4A-I
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 4A1
• NCBI Official Symbol: EIF4A1
• NCBI Protein Information: eukaryotic initiation factor 4A-I
• UniProt Protein Name: Eukaryotic initiation factor 4A-I
• Protein Family: Eukaryotic initiation factor
• UniProt Gene Name: EIF4A1
• UniProt Synonym Gene Names: eIF-4A-I; eIF4A-I

Uniprot Description

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon ().