Bovine euglobulin (EL) ELISA Kit | EL elisa kit

Bovine euglobulin(EL) ELISA Kit

Cat. Num. AAA2609068

• Gene Names: LIPG; EL; EDL; PRO719
• Reactivity: Bovine
• Synonyms: euglobulin (EL); Bovine euglobulin(EL) ELISA Kit; EL elisa kit

• Reactivity: Bovine
• Sequence Length: 500

• Samples: Bovine serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 200 ng/mL-3.12 ng/mL
• Sensitivity: Up to 0.6 ng/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EL elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Bovine EL monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 5174497
• NCBI GeneID: 9388
• NCBI Accession #: NP_006024.1
• NCBI GenBank Nucleotide #: NM_006033.3
• Molecular Weight: 39,362 Da
• NCBI Official Full Name: endothelial lipase isoform 1
• NCBI Official Synonym Full Names: lipase G, endothelial type
• NCBI Official Symbol: LIPG
• NCBI Official Synonym Symbols: EL; EDL; PRO719
• NCBI Protein Information: endothelial lipase
• UniProt Protein Name: Endothelial lipase
• UniProt Gene Name: LIPG
• UniProt Synonym Gene Names: EDL; EL
• UniProt Entry Name: LIPE_HUMAN

NCBI Description

The protein encoded by this gene has substantial phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. This protein is designated a member of the TG lipase family by its sequence and characteristic lid region which provides substrate specificity for enzymes of the TG lipase family. [provided by RefSeq, Jul 2008]

Uniprot Description

LIPG: Has phospholipase and triglyceride lipase activities. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins. Binds heparin. Belongs to the AB hydrolase superfamily. Lipase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.1.3; Lipid Metabolism - glycerolipid; Phospholipase; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 18q21.1

Cellular Component: cell surface; early endosome; extracellular region; extracellular space; Golgi apparatus

Molecular Function: lipoprotein lipase activity; phospholipase A1 activity; phospholipase activity

Biological Process: cholesterol homeostasis; lipid digestion; lipid metabolic process; phospholipid homeostasis; positive regulation of cholesterol transport; reverse cholesterol transport