Bovine Corin (Atrial natriuretic peptide-converting enzyme) ELISA Kit | Corin elisa kit

Bovine Corin (Atrial natriuretic peptide-converting enzyme) ELISA Kit

Cat. Num. AAA751401

• Gene Names: CORIN; CRN; ATC2; Lrp4; PEE5; TMPRSS10
• Reactivity: Bovine
• Synonyms: Corin (Atrial natriuretic peptide-converting enzyme); Bovine Corin (Atrial natriuretic peptide-converting enzyme) ELISA Kit; Corin elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Intended Uses: This Corin ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine Corin. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This Corin ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine Corin. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Corin elisa kit

Principle of the Assay: Corin ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Corin antibody and an Corin-HRP conjugate. The assay sample and buffer are incubated together with Corin-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Corin concentration since Corin from samples and Corin-HRP conjugate compete for the anti-Corin antibody binding site. Since the number of sites is limited, as more sites are occupied by Corin from the sample, fewer sites are left to bind Corin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Corin concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4884872
• NCBI GeneID: 10699
• Molecular Weight: 113,297 Da
• NCBI Official Full Name: corin
• NCBI Official Synonym Full Names: corin, serine peptidase
• NCBI Official Symbol: CORIN
• NCBI Official Synonym Symbols: CRN; ATC2; Lrp4; PEE5; TMPRSS10
• NCBI Protein Information: atrial natriuretic peptide-converting enzyme; pro-ANP-convertase; pro-ANP-converting enzyme; transmembrane protease serine 10; heart-specific serine proteinase ATC2
• UniProt Protein Name: Atrial natriuretic peptide-converting enzyme
• Protein Family: Atrial natriuretic peptide-converting enzyme
• UniProt Gene Name: CORIN
• UniProt Synonym Gene Names: CRN; TMPRSS10
• UniProt Entry Name: CORIN_HUMAN

NCBI Description

This gene encodes a member of the type II transmembrane serine protease class of the trypsin superfamily. Members of this family are composed of multiple structurally distinct domains. The encoded protein converts pro-atrial natriuretic peptide to biologically active atrial natriuretic peptide, a cardiac hormone that regulates blood volume and pressure. This protein may also function as a pro-brain-type natriuretic peptide convertase. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]

Uniprot Description

CORIN: Serine-type endopeptidase involved in atrial natriuretic peptide hormone (NPPA) processing. Converts through proteolytic cleavage the non-functional propeptide NPPA into the active hormone, thereby regulating blood pressure in heart and promoting natriuresis, diuresis and vasodilation. Proteolytic cleavage of pro-NPPA also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Also acts as a regulator of sodium reabsorption in kidney. May also process pro-NPPB the B-type natriuretic peptide. Defects in CORIN are the cause of pre-eclampsia/eclampsia 5 (PEE5); also known as gestational proteinuric hypertension. Preeclampsia is a pregnancy-associated disease with maternal symptoms but placental origin. Unlike most other human disorders, it impacts 2 individuals, the mother and her child, both of whom can be severely affected. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.21.-; Protease; Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 4p12

Cellular Component: cell surface; integral to plasma membrane; integral to membrane; plasma membrane; extracellular region

Molecular Function: serine-type endopeptidase activity

Biological Process: regulation of blood pressure; peptide hormone processing; female pregnancy; proteolysis; regulation of systemic arterial blood pressure by atrial natriuretic peptide

Disease: Preeclampsia/eclampsia 5