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Typical Testing Data/Standard Curve (for reference only)

Bovine C-X-C motif chemokine 2 (CXCL2) ELISA Kit | CXCL2 elisa kit

Bovine C-X-C motif chemokine 2 (CXCL2) ELISA Kit

Gene Names
CXCL2; GRO2; GROb; MIP2; MIP2A; SCYB2; MGSA-b; MIP-2a; CINC-2a
Reactivity
Bovine
Synonyms
C-X-C motif chemokine 2 (CXCL2); Bovine C-X-C motif chemokine 2 (CXCL2) ELISA Kit; CXCL2 elisa kit
Ordering
For Research Use Only!
Reactivity
Bovine
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/mL.
Intended Uses
This CXCL-2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine CXCL-2. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CXCL2 elisa kit
Principle of the assay: CXCL-2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CXCL-2 antibody and an CXCL-2-HRP conjugate. The assay sample and buffer are incubated together with CXCL-2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CXCL-2 concentration since CXCL-2 from samples and CXCL-2-HRP conjugate compete for the anti-CXCL-2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CXCL-2 from the sample, fewer sites are left to bind CXCL-2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CXCL-2 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CXCL2 elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,389 Da
NCBI Official Full Name
C-X-C motif chemokine 2
NCBI Official Synonym Full Names
chemokine (C-X-C motif) ligand 2
NCBI Official Symbol
CXCL2
NCBI Official Synonym Symbols
GRO2; GROb; MIP2; MIP2A; SCYB2; MGSA-b; MIP-2a; CINC-2a
NCBI Protein Information
C-X-C motif chemokine 2; gro-beta; MGSA beta; MIP2-alpha; GRO2 oncogene; growth-regulated protein beta; macrophage inflammatory protein 2-alpha; melanoma growth stimulatory activity beta
UniProt Protein Name
C-X-C motif chemokine 2
Protein Family
UniProt Gene Name
CXCL2
UniProt Synonym Gene Names
GRO2; GROB; MIP2A; SCYB2; Gro-beta; MIP2-alpha; HSF
UniProt Entry Name
CXCL2_HUMAN

NCBI Description

This antimicrobial gene is part of a chemokine superfamily that encodes secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CXC subfamily, is expressed at sites of inflammation and may suppress hematopoietic progenitor cell proliferation. [provided by RefSeq, Sep 2014]

Uniprot Description

CXCL2: Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; chemokine activity; CXCR chemokine receptor binding

Biological Process: G-protein coupled receptor protein signaling pathway; response to molecule of bacterial origin; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; immune response; chemotaxis; inflammatory response; regulation of cell proliferation

Research Articles on CXCL2

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Product Notes

The Bovine CXCL2 cxcl2 (Catalog #AAA7240964) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7240964 ELISA Kit recognizes Bovine CXCL2. It is sometimes possible for the material contained within the vial of "C-X-C motif chemokine 2 (CXCL2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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