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Typical Testing Data/Standard Curve (for reference only)

Bovine Bifunctional aminoacyl-tRNA synthetase (EPRS) ELISA Kit | EPRS elisa kit

Bovine Bifunctional aminoacyl-tRNA synthetase (EPRS) ELISA Kit

Gene Names
eprs; wu:fb06c03; wu:fb38d08; wu:ft34d10
Reactivity
Bovine
Synonyms
Bifunctional aminoacyl-tRNA synthetase (EPRS); Bovine Bifunctional aminoacyl-tRNA synthetase (EPRS) ELISA Kit; EPRS elisa kit
Ordering
For Research Use Only!
Reactivity
Bovine
Specificity
This assay has high sensitivity and excellent specificity for detection of EPRS. No significant cross-reactivity or interference between EPRS and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between EPRS and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for EPRS elisa kit
Intended Uses: This EPRS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine EPRS. This ELISA kit for research use only!

Principle of the Assay: EPRS ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-EPRS antibody and an EPRS-HRP conjugate. The assay sample and buffer are incubated together with EPRS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EPRS concentration since EPRS from samples and EPRS-HRP conjugate compete for the anti-EPRS antibody binding site. Since the number of sites is limited, as more sites are occupied by EPRS from the sample, fewer sites are left to bind EPRS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EPRS concentration in each sample is interpolated from this standard curve.
Product Categories/Family for EPRS elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
bifunctional aminoacyl-tRNA synthetase
NCBI Official Synonym Full Names
glutamyl-prolyl-tRNA synthetase
NCBI Official Symbol
eprs
NCBI Official Synonym Symbols
wu:fb06c03; wu:fb38d08; wu:ft34d10
NCBI Protein Information
bifunctional aminoacyl-tRNA synthetase

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Product Notes

The Bovine EPRS (Catalog #AAA7214380) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7214380 ELISA Kit recognizes Bovine EPRS. It is sometimes possible for the material contained within the vial of "Bifunctional aminoacyl-tRNA synthetase (EPRS), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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