Botulinum neurotoxin type B Recombinant Protein | botB recombinant protein

Recombinant Clostridium botulinum Botulinum neurotoxin type B

Cat. Num. AAA1107461

• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Botulinum neurotoxin type B; Recombinant Clostridium botulinum Botulinum neurotoxin type B; Bontoxilysin-B; botB recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-427. Partial
• Sequence: MPVTINNFNYNDPIDNNNIIMMEPPFARGTGRYYKAFKITDRIWIIPERYTFGYKPEDFNKSSGIFNRDVCEYYDPDYLNTNDKKNIFLQTMIKLFNRIKSKPLGEKLLEMIINGIPYLGDRRVPLEEFNTNIASVTVNKLISNPGEVERKKGIFANLIIFGPGPVLNENETIDIGIQNHFASREGFGGIMQMKFCPEYVSVFNNVQENKGASIFNRRGYFSDPALILMHELIHVLHGLYGIKVDDLPIVPNEKKFFMQSTDAIQAEELYTFGGQDPSIITPSTDKSIYDKVLQNFRGIVDRLNKVLVCISDPNININIYKNKFKDKYKFVEDSEGKYSIDVESFDKLYKSLMFGFTETNIAENYKIKTRASYFSDSLPPVKIKNLLDNEIYTIEEGFNISDKDMEKEYRGQNKAINKQAYEEISKE

• Production Note: Special Offer: The E Coli, Yeast host-expressed protein is manufactured from a stock plasmid containing the protein gene. E Coli, Yeasthost-expressed protein is stocked in different unit sizes ranging from as small as 10 ug to as large as 1 mg. Bulk inventory is also available. The E Coli, Yeast host-expressed protein has been ordered over and over again by researchers and has stood the test of time as both a robust protein and important target for the research community. It is part of our new program to make our most popular protein targets and corresponding hosts available in expanded unit sizes and with a quick processing time. Select E Coli, Yeast host-expressed protein for the fastest delivery among all hosts. Please contact our technical support team or email to [email protected] for more details.
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-Page

Related Product Information for botB recombinant protein

Botulinum toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that cleaves the '76-Gln-|-Phe-77' bond of synaptobrevin-2.

References

Molecular cloning of the Clostridium botulinum structural gene encoding the type B neurotoxin and determination of its entire nucleotide sequence.Whelan S.M., Elmore M.J., Bodsworth N.J., Brehm J.K., Atkinson T., Minton N.P.Appl. Environ. Microbiol. 58:2345-2354(1992) Szabo E.A., Pemberton J.M., Desmarchelier P.M. Gene probes for identification of the botulinal neurotoxin gene and specific identification of neurotoxin types B, E, and F.Campbell K.D., Collins M.D., East A.K.J. Clin. Microbiol. 31:2255-2262(1993) Botulinum neurotoxin type B (strain 657) partial sequence and similarity with tetanus toxin.Dasgupta B.R., Datta A.Biochimie 70:811-817(1988) Botulinum neurotoxins are zinc proteins.Schiavo G., Rossetto O., Santucci A., Dasgupta B.R., Montecucco C.J. Biol. Chem. 267:23479-23483(1992) Tetanus and botulinum-B neurotoxins block neurotransmitter release by proteolytic cleavage of synaptobrevin.Schiavo G., Benfenati F., Poulain B., Rossetto O., de Laureto P.P., Dasgupta B.R., Montecucco C.Nature 359:832-835(1992)

NCBI and Uniprot Product Information

• NCBI GI #: 399134
• UniProt Accession #: P10844; P10843
• Molecular Weight: 51.3 kDa
• NCBI Official Full Name: Botulinum neurotoxin type B
• UniProt Protein Name: Botulinum neurotoxin type B
• Protein Family: Botulinum neurotoxin
• UniProt Gene Name: botB
• UniProt Synonym Gene Names: BoNT/B
• UniProt Entry Name: BXB_CLOBO

Uniprot Description

Botulinum toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that cleaves the '76-Gln-|-Phe-77' bond of synaptobrevin-2.