SDS-Page
BOLA1 recombinant protein
BOLA1, 21-137aa, Human, His tag, E Coli
Gene Names
BOLA1; CGI-143
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
BOLA1; 21-137aa; Human; His tag; E Coli; BolA-like protein 1; CGI-143; BOLA1 recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol, 1mM DTT
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSQGSAGSG AIGPVEAAIR TKLEEALSPE VLELRNESGG HAVPPGSETH FRVAVVSSRF EGLSPLQRHR LVHAALAEEL GGPVHALAIQ ARTPAQWREN SQLDTSPPCL GGNKKTLGTP
Sequence Length
137
Applicable Applications for BOLA1 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for BOLA1 recombinant protein
BOLA1 belongs to the bolA/yrbA family. BolA proteins (hBolA1, hBolA2, and hBolA3) are novel non-classical secreted proteins identified with bioinformatics and molecular biology experiments. The three BolA fusion proteins with c-Myc tag could be secreted into the culture medium of the transfected Cos-7 cells, although they could not be colocalized with Golgi apparatus. And the secretion of three BolA proteins could not be inhibited after BFA treatment. Recombinant human BOLA1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
References
Zhou YB, Cao JB, et al. (2008). Mol Cell Biochem. 317 (1-2):61-8.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14kDa (140aa), confirmed by MALDI-TOF
NCBI Official Full Name
bolA-like protein 1
NCBI Official Synonym Full Names
bolA family member 1
NCBI Official Symbol
BOLA1
NCBI Official Synonym Symbols
CGI-143
NCBI Protein Information
bolA-like protein 1
UniProt Protein Name
BolA-like protein 1
Protein Family
UniProt Gene Name
BOLA1
Uniprot Description
Acts as a mitochondrial iron-sulfur (Fe-S) cluster assembly factor that facilitates (Fe-S) cluster insertion into a subset of mitochondrial proteins (). Probably acts together with the monothiol glutaredoxin GLRX5 (PubMed:27532772). May protect cells against oxidative stress (PubMed:22746225).
Research Articles on BOLA1
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Product Notes
The BOLA1 bola1 (Catalog #AAA205376) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's BOLA1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the BOLA1 bola1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSQGSAGSG AIGPVEAAIR TKLEEALSPE VLELRNESGG HAVPPGSETH FRVAVVSSRF EGLSPLQRHR LVHAALAEEL GGPVHALAIQ ARTPAQWREN SQLDTSPPCL GGNKKTLGTP. It is sometimes possible for the material contained within the vial of "BOLA1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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