Typical Testing Data/Standard Curve (for reference only)
Mouse Bone Morphogenetic Protein Receptor 1B ELISA Kit | BMPR1B elisa kit
Mouse Bone Morphogenetic Protein Receptor 1B ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: BMPR1B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BMPR1B antibody and an BMPR1B-HRP conjugate. The assay sample and buffer are incubated together with BMPR1B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BMPR1B concentration since BMPR1B from samples and BMPR1B-HRP conjugate compete for the anti-BMPR1B antibody binding site. Since the number of sites is limited, as more sites are occupied by BMPR1B from the sample, fewer sites are left to bind BMPR1B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BMPR1B concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. The ligands of this receptor are BMPs, which are members of the TGF-beta superfamily. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. Mutations in this gene have been associated with primary pulmonary hypertension. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]
Uniprot Description
BMPR1B: a kinase of the STKR family. A type I cell-surface receptor for bone morphogenetic protein. Expressed at ighest level in the prostate. May be upregulated by androgen stimulation in cells which expresses the androgen receptor.
Protein type: Protein kinase, TKL; EC 2.7.11.30; Kinase, protein; Membrane protein, integral; Protein kinase, Ser/Thr (receptor); TKL group; STKR family; Type1 subfamily
Chromosomal Location of Human Ortholog: 4q22-q24
Cellular Component: integral to plasma membrane; plasma membrane; receptor complex
Molecular Function: protein serine/threonine kinase activity; protein binding; transforming growth factor beta receptor activity, type I; metal ion binding; SMAD binding; ATP binding; transmembrane receptor protein serine/threonine kinase activity; receptor signaling protein serine/threonine kinase activity
Biological Process: chondrocyte development; positive regulation of bone mineralization; limb morphogenesis; protein amino acid phosphorylation; BMP signaling pathway; positive regulation of osteoblast differentiation; ovulation cycle; dorsal/ventral pattern formation; eye development; retina development in camera-type eye; proteoglycan biosynthetic process; ovarian cumulus expansion; inflammatory response; positive regulation of cell differentiation; skeletal development; cartilage condensation; retinal ganglion cell axon guidance
Disease: Brachydactyly, Type A2; Chondrodysplasia, Acromesomelic, With Genital Anomalies
Research Articles on BMPR1B
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Product Notes
The Mouse BMPR1B bmpr1b (Catalog #AAA755116) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA755116 ELISA Kit recognizes Mouse BMPR1B. It is sometimes possible for the material contained within the vial of "Bone Morphogenetic Protein Receptor 1B, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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