YWHAB blocking peptide

YWHAB Antibody (N-term) Blocking peptide

• Gene Names: YWHAB; HS1; GW128; YWHAA; KCIP-1; HEL-S-1
• Synonyms: YWHAB; YWHAB Antibody (N-term) Blocking peptide; 14-3-3 protein beta/alpha; Protein 1054; Protein kinase C inhibitor protein 1; KCIP-1; N-terminally processed; YWHAB blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the 14-3-3 region of human YWHAB (14-3-3 protein beta/alpha). A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 246

• Cellular Location: Cytoplasm. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for YWHAB blocking peptide

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2.

NCBI and Uniprot Product Information

• NCBI GI #: 1345590
• NCBI GeneID: 7529
• UniProt Accession #: P31946; A8K9K2; E1P616
• Molecular Weight: 27,850 Da
• NCBI Official Full Name: 14-3-3 protein beta/alpha
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta
• NCBI Official Symbol: YWHAB
• NCBI Official Synonym Symbols: HS1; GW128; YWHAA; KCIP-1; HEL-S-1
• NCBI Protein Information: 14-3-3 protein beta/alpha
• UniProt Protein Name: 14-3-3 protein beta/alpha
• Protein Family: 14-3-3 protein
• UniProt Gene Name: YWHAB
• UniProt Synonym Gene Names: KCIP-1
• UniProt Entry Name: 1433B_HUMAN

NCBI Description

This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

14-3-3 beta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 20q13.1

Cellular Component: cytoplasm; cytoplasmic vesicle membrane; cytosol; focal adhesion; membrane; perinuclear region of cytoplasm

Molecular Function: enzyme binding; histone deacetylase binding; phosphoprotein binding; phosphoserine binding; protein binding; protein domain specific binding

Biological Process: cytoplasmic sequestering of protein; MAPKKK cascade; negative regulation of G-protein coupled receptor protein signaling pathway; negative regulation of protein amino acid dephosphorylation; positive regulation of catalytic activity; regulation of mRNA stability