WWOX blocking peptide

WWOX Blocking Peptide

Cat. Num. AAA9623807

• Gene Names: WWOX; FOR; WOX1; EIEE28; FRA16D; SCAR12; HHCMA56; PRO0128; SDR41C1; D16S432E
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: WWOX; WWOX Blocking Peptide; 5330426P09Rik; 9030416C10Rik; Aberrant WW domain-containing oxidoreductase; D16S432E; EC 1.1.1.-; EIEE28; FOR; FRA16D; Fragile site FRA16D oxidoreductase; Fragile site FRA16D Oxireductase; HHCMA56; MGC55975; PRO0128; Putative oxidoreductase; SCAR12; SDR41C1; Short chain dehydrogenase/reductase family 41C; member 1; WOX1; WW domain containing oxidoreductase; WW domain-containing oxidoreductase; WW domain-containing protein WWOX; wwox; WWOX_HUMAN; zgc:55975; WWOX blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for WWOX blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for WWOX blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 635172870
• NCBI GeneID: 51741
• NCBI Accession #: NP_001278926.1
• NCBI GenBank Nucleotide #: NM_001291997.1
• UniProt Accession #: Q9NZC7; Q5MYT5; Q96KM3; Q96RF2; Q9BTT8; Q9NPC9; Q9NRF4; Q9NRF5; Q9NRF6; Q9NRK1; Q9NZC5; A8K323
• Molecular Weight: 23,868 Da
• NCBI Official Full Name: WW domain-containing oxidoreductase isoform 4
• NCBI Official Synonym Full Names: WW domain containing oxidoreductase
• NCBI Official Symbol: WWOX
• NCBI Official Synonym Symbols: FOR; WOX1; EIEE28; FRA16D; SCAR12; HHCMA56; PRO0128; SDR41C1; D16S432E
• NCBI Protein Information: WW domain-containing oxidoreductase; WW domain-containing protein WWOX; fragile site FRA16D oxidoreductase; short chain dehydrogenase/reductase family 41C, member 1
• UniProt Protein Name: WW domain-containing oxidoreductase
• Protein Family: WW domain-containing oxidoreductase
• UniProt Gene Name: WWOX
• UniProt Synonym Gene Names: FOR; WOX1
• UniProt Entry Name: WWOX_HUMAN

NCBI Description

This gene encodes a member of the short-chain dehydrogenases/reductases (SDR) protein family. This gene spans the FRA16D common chromosomal fragile site and appears to function as a tumor suppressor gene. Expression of the encoded protein is able to induce apoptosis, while defects in this gene are associated with multiple types of cancer. Disruption of this gene is also associated with autosomal recessive spinocerebellar ataxia 12. Disruption of a similar gene in mouse results in impaired steroidogenesis, additionally suggesting a metabolic function for the protein. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

WWOX: an enzyme which contains 2 WW domains and a short-chain dehydrogenase/reductase domain (SRD). Expressed at high levels in hormonally regulated tissues such as testis, ovary, and prostate. Its SRD domain suggest a role for this gene in steroid metabolism. Shown to be an essential mediator of tumor necrosis factor-alpha-induced apoptosis in the mouse. May play a similar role in the human. May function as a suppressor of tumor growth. Alternative splicing of this gene generates 7 isoforms.

Protein type: Tumor suppressor; Oxidoreductase; EC 1.1.1.-; Apoptosis

Chromosomal Location of Human Ortholog: 16q23

Cellular Component: Golgi apparatus; microvillus; mitochondrion; cytoplasm; plasma membrane; nucleus; cytosol

Molecular Function: protein dimerization activity; protein binding; enzyme binding; oxidoreductase activity; coenzyme binding; cofactor binding

Biological Process: osteoblast differentiation; steroid metabolic process; Wnt receptor signaling pathway; negative regulation of Wnt receptor signaling pathway; skeletal morphogenesis; positive regulation of transcription from RNA polymerase II promoter

Disease: Epileptic Encephalopathy, Early Infantile, 28