VPS4B blocking peptide

VPS4B Antibody (C-term) Blocking peptide

• Gene Names: VPS4B; MIG1; SKD1; SKD1B; VPS4-2
• Synonyms: VPS4B; VPS4B Antibody (C-term) Blocking peptide; Vacuolar protein sorting-associated protein 4B; Cell migration-inducing gene 1 protein; Suppressor of K(+) transport growth defect 1; Protein SKD1; SKD1; VPS42; VPS4B blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of VPS4B. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 444

• Cellular Location: Prevacuolar compartment membrane; Peripheral membrane protein. Late endosome membrane; Peripheral membrane protein. Note: Membrane-associated in the prevacuolar endosomal compartment. Localized in HIV-1 particles purified from acutely infected cells
• Tissue Location: Ubiquitously expressed.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for VPS4B blocking peptide

Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses).

NCBI and Uniprot Product Information

• NCBI GI #: 41019529
• NCBI GeneID: 9525
• UniProt Accession #: O75351; Q69HW4; Q9GZS7
• Molecular Weight: 49,302 Da
• NCBI Official Full Name: Vacuolar protein sorting-associated protein 4B
• NCBI Official Synonym Full Names: vacuolar protein sorting 4 homolog B
• NCBI Official Symbol: VPS4B
• NCBI Official Synonym Symbols: MIG1; SKD1; SKD1B; VPS4-2
• NCBI Protein Information: vacuolar protein sorting-associated protein 4B
• UniProt Protein Name: Vacuolar protein sorting-associated protein 4B
• Protein Family: Vacuolar protein sorting-associated protein
• UniProt Gene Name: VPS4B
• UniProt Synonym Gene Names: SKD1; VPS42; Protein SKD1
• UniProt Entry Name: VPS4B_HUMAN

NCBI Description

The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. Mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be a yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 18; the gene for the other resides on chromosome 16. [provided by RefSeq, Jul 2008]

Uniprot Description

VPS4B: Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Belongs to the AAA ATPase family.

Protein type: EC 3.6.4.6; Vesicle

Chromosomal Location of Human Ortholog: 18q21.33

Cellular Component: centrosome; cytoplasm; cytosol; endosome; endosome membrane; nucleus; spindle pole

Molecular Function: ATPase activity; ATPase activity, coupled; identical protein binding; microtubule-severing ATPase activity; protein binding; protein C-terminus binding; protein homodimerization activity

Biological Process: cell separation during cytokinesis; cytoplasmic microtubule organization and biogenesis; endosome to lysosome transport via multivesicular body sorting pathway; endosome transport; mitotic metaphase plate congression; nuclear organization and biogenesis; positive regulation of viral reproduction; protein depolymerization; regulation of viral reproduction; release of virus from host; response to lipid; ubiquitin-dependent protein catabolic process via the multivesicular body pathway; vacuole organization and biogenesis; viral infectious cycle