VDU1 blocking peptide

VDU1 Antibody (C-term) Blocking Peptide

• Gene Names: USP33; VDU1
• Synonyms: VDU1; VDU1 Antibody (C-term) Blocking Peptide; Ubiquitin carboxyl-terminal hydrolase 33; Deubiquitinating enzyme 33; Ubiquitin thioesterase 33; Ubiquitin-specific-processing protease 33; VHL-interacting deubiquitinating enzyme 1; hVDU1; USP33; KIAA1097; VDU1 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human VDU1-II. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Lyophilized with 100% acetonitrile and is supplied as a powder.
• Sequence Length: 942

• Reconstitution: Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for VDU1 blocking peptide

Type 2 iodothyronine deiodinase (D2) is an integral membrane selenoenzyme that stimulates the pro-hormone thyroxine (T4) and supplies the majority of the 3,5,3?-triiodothyronine (T3) essential for brain development.1 T4 catalysis accelerates selective conjugation to ubiquitin and thereby renders D2 inactive, a posttranslational feedback mechanism used to maintain acceptable T3 levels.2,3 Ub-D2 was the first recognized substrate for von Hippel?Lindau protein?interacting (pVHL-interacting) deubiquitinating enzyme-1 (VDU1).4 VDU proteins colocalize with D2 in the endoplasmic reticulum, and their coexpression provides D2 resistance to degradation. VDU1 expression is substantially upregulated in brown adipocytes by norepinephrine or cold exposure, further amplifying D2 activity. VDU1 and VDU2 are coexpressed with D2 in many human tissues, including brain, heart, and skeletal muscle, suggesting potential roles in neurological development, cardiac function, and energy management, in addition to thermal homeostasis.VDU1- or VDU2-catalyzed deubiquitination recycles inactive Ub-D2 to its active deubiquitinated form, circumventing the proteasomal degradation pathway. Thus, Ub-D2 can be either reactivated or degraded, with the balance between these two processes influenced by VDU activity.VDU1-catalyzed D2 deubiquitination may be an important participant in the adaptive mechanism that regulates thyroid hormone action. The reversible ubiquitination-dependent mechanism regulating D2 activity permits highly responsive control of thyroid hormone activation.5,6

NCBI and Uniprot Product Information

• NCBI GI #: 42516567
• NCBI GeneID: 23032
• NCBI Accession #: NP_055832.3; NP_963918.1; NP_963920.1
• NCBI GenBank Nucleotide #: NM_015017.4
• UniProt Accession #: Q8TEY7; A6QNM7; Q8TEY6
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 33 isoform 1
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 33
• NCBI Official Symbol: USP33
• NCBI Official Synonym Symbols: VDU1
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 33
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 33
• UniProt Gene Name: USP33
• UniProt Synonym Gene Names: KIAA1097; VDU1
• UniProt Entry Name: UBP33_HUMAN

NCBI Description

This gene encodes a deubiquinating enzyme important in a variety of processes, including Slit-dependent cell migration and beta-2 adrenergic receptor signaling. The protein is negatively regulated through ubiquitination by von Hippel-Lindau tumor protein (VHL). Alternative splicing results in multiple transcript variants and protein isoforms. [provided by RefSeq, Jun 2012]