USP2 blocking peptide

USP2 Blocking Peptide (C-term)

• Gene Names: USP2; USP9; UBP41
• Synonyms: USP2; USP2 Blocking Peptide (C-term); Ubiquitin carboxyl-terminal hydrolase 2; 41 kDa ubiquitin-specific protease; Deubiquitinating enzyme 2; Ubiquitin thioesterase 2; Ubiquitin-specific-processing protease 2; UBP41; USP2 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human USP2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder.
• Sequence Length: 362

• Reconstitution: Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Cellular Location: Cytoplasm {ECO:0000250|UniProtKB:O88623}. Cytoplasm, perinuclear region {ECO:0000250|UniProtKB:O88623} Note=Localizes in the spermatid head in late-elongating spermatids in the thin area between the outer acrosomal membrane and the plasma membrane. {ECO:0000250|UniProtKB:Q5U349}
• Peptide ID: 3062308
• Tissue Location: Expressed in mesangial cells of the kidney and in different types of glomerulonephritides (at protein level)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for USP2 blocking peptide

Hydrolase that deubiquitinates polyubiquitinated target proteins such as MDM2, MDM4 and CCND1. Isoform 1 and isoform 4 possess both ubiquitin-specific peptidase and isopeptidase activities. Deubiquitinates MDM2 without reversing MDM2-mediated p53/TP53 ubiquitination and thus indirectly promotes p53/TP53 degradation and limits p53 activity. Has no deubiquitinase activity against p53/TP53. Prevents MDM2-mediated degradation of MDM4. Plays a role in the G1/S cell-cycle progression in normal and cancer cells. Plays a role in the regulation of myogenic differentiation of embryonic muscle cells. Regulates the circadian clock by modulating its intrinsic circadian rhythm and its capacity to respond to external cues. Associates with clock proteins and deubiquitinates core clock component PER1 but does not affect its overall stability. Regulates the nucleocytoplasmic shuttling and nuclear retention of PER1 and its repressive role on the clock transcription factors CLOCK and ARNTL/BMAL1 (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 344313155
• NCBI GeneID: 9099
• NCBI Accession #: NP_001230688.1
• NCBI GenBank Nucleotide #: NM_001243759.1
• UniProt Accession #: O75604; Q8IUM2; Q8IW04; Q96MB9; Q9BQ21; B0YJB8; E9PPM2
• Molecular Weight: 45,241 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 2 isoform c
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 2
• NCBI Official Symbol: USP2
• NCBI Official Synonym Symbols: USP9; UBP41
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 2
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 2
• Protein Family: Protein
• UniProt Gene Name: USP2
• UniProt Synonym Gene Names: UBP41

NCBI Description

This gene encodes a member of the family of de-ubiquitinating enzymes, which belongs to the peptidase C19 superfamily. The encoded protein is a ubiquitin-specific protease which is required for TNF-alpha (tumor necrosis factor alpha) -induced NF-kB (nuclear factor kB) signaling. This protein deubiquitinates polyubiquitinated target proteins such as fatty acid synthase, murine double minute 2 (MDM2), MDM4/MDMX and cyclin D1. MDM2 and MDM4 are negative regulators of the p53 tumor suppressor and cyclin D1 is required for cell cycle G1/S transition. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

Uniprot Description

USP2: Hydrolase that deubiquitinates polyubiquitinated target proteins such as MDM2, MDM4 and CCND1. Isoform 1 and isoform 4 possess both ubiquitin-specific peptidase and isopeptidase activities. Deubiquitinates MDM2 without reversing MDM2-mediated p53/TP53 ubiquitination and thus indirectly promotes p53/TP53 degradation and limits p53 activity. Has no deubiquitinase activity against p53/TP53. Prevents MDM2-mediated degradation of MDM4. Plays a role in the G1/S cell-cycle progression in normal and cancer cells. Plays a role in the regulation of myogenic differentiation of embryonic muscle cells. Homooligomer. Found in trimeric complex with MDM2 and MDM4 and UPB2. Interacts with CCND1; the interaction is direct and promotes its stabilization by antagonizing ubiquitin-dependent degradation. Interacts (via N-terminus and C- terminus) with MDM2. Interacts with MDM4. Down-regulated by cisplatin. Expressed in mesangial cells of the kidney and in different types of glomerulonephritides. Cleavage is inhibited by ubiquitin in a dosage- dependent manner. Cleavage is blocked by ubiquitin aldehyde. Belongs to the peptidase C19 family. USP2 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.19.12; Protease; Ubiquitin-specific protease

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: nucleoplasm

Molecular Function: cyclin binding; cysteine-type endopeptidase activity; protein binding; ubiquitin protein ligase binding; ubiquitin-specific protease activity

Biological Process: circadian behavior; circadian regulation of gene expression; entrainment of circadian clock by photoperiod; locomotor rhythm; negative regulation of transcription from RNA polymerase II promoter; positive regulation of mitotic cell cycle; protein deubiquitination; protein stabilization