TRIM33 blocking peptide

TRIM33 Antibody (C-term) Blocking peptide

• Gene Names: TRIM33; ECTO; PTC7; RFG7; TF1G; TIF1G; TIFGAMMA; TIF1GAMMA
• Synonyms: TRIM33; TRIM33 Antibody (C-term) Blocking peptide; E3 ubiquitin-protein ligase TRIM33; 632-; Ectodermin homolog; RET-fused gene 7 protein; Protein Rfg7; Transcription intermediary factor 1-gamma; TIF1-gamma; Tripartite motif-containing protein 33; KIAA1113; RFG7; TIF1G; TRIM33 blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 1127

• Cellular Location: Nucleus. Note: In discrete nuclear dots resembling nuclear bodies.
• Tissue Location: Expressed in stem cells at the bottom of the crypts of the colon (at protein level). Expressed in colon adenomas and adenocarcinomas (at protein level). Expressed in brain, lung, liver, spleen, thymus, prostate, kidney, testis, heart, placenta, pancreas, small intestine, ovary, colon, skeletal muscle and hematopoietic progenitors
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for TRIM33 blocking peptide

Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to PubMed:16751102, does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor (By similarity). Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF- beta/BMP signaling cascade).

NCBI and Uniprot Product Information

• NCBI GI #: 313104270
• NCBI GeneID: 51592
• UniProt Accession #: Q9UPN9; O95855; Q5TG72; Q5TG73; Q5TG74; Q9C017; Q9UJ79
• Molecular Weight: 120,541 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase TRIM33
• NCBI Official Synonym Full Names: tripartite motif containing 33
• NCBI Official Symbol: TRIM33
• NCBI Official Synonym Symbols: ECTO; PTC7; RFG7; TF1G; TIF1G; TIFGAMMA; TIF1GAMMA
• NCBI Protein Information: E3 ubiquitin-protein ligase TRIM33
• UniProt Protein Name: E3 ubiquitin-protein ligase TRIM33
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: TRIM33
• UniProt Synonym Gene Names: KIAA1113; RFG7; TIF1G; Protein Rfg7; TIF1-gamma
• UniProt Entry Name: TRI33_HUMAN

NCBI Description

The protein encoded by this gene is thought to be a transcriptional corepressor. However, molecules that interact with this protein have not yet been identified. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Three alternatively spliced transcript variants for this gene have been described, however, the full-length nature of one variant has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

TIF1G: an atypical protein kinase that functions as a transcriptional repressor. Translocation with RET generates the TRIM33/RET (PTC7) oncogene. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Two splice variant isoforms have been described.

Protein type: Kinase, protein; Ubiquitin ligase; Ligase; EC 6.3.2.19; Ubiquitin conjugating system; Transcription, coactivator/corepressor; EC 6.3.2.-; Protein kinase, atypical; ATYPICAL group; TIF1 family

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: intracellular membrane-bound organelle; nucleoplasm; nucleus

Molecular Function: protein binding; ubiquitin-protein ligase activity

Biological Process: negative regulation of BMP signaling pathway; negative regulation of transcription from RNA polymerase II promoter; protein ubiquitination; regulation of transforming growth factor beta receptor signaling pathway

Disease: Thyroid Carcinoma, Papillary