TRAF7 blocking peptide

TRAF7 Blocking Peptide

Cat. Num. AAA9618555

• Gene Names: TRAF7; RFWD1; RNF119
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: TRAF7; TRAF7 Blocking Peptide; E3 ubiquitin protein ligase TRAF7; E3 ubiquitin-protein ligase TRAF7; RFWD1; Ring finger and WD repeat domain 1; RING finger and WD repeat-containing protein 1; RING finger protein 119; RNF119; TNF receptor associated factor 7; TNF receptor-associated factor 7; TRAF7_HUMAN; TRAF7 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for TRAF7 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for TRAF7 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 54036486
• NCBI GeneID: 84231
• UniProt Accession #: Q6Q0C0; Q9H073
• Molecular Weight: 670
• NCBI Official Full Name: E3 ubiquitin-protein ligase TRAF7
• NCBI Official Synonym Full Names: TNF receptor-associated factor 7, E3 ubiquitin protein ligase
• NCBI Official Symbol: TRAF7
• NCBI Official Synonym Symbols: RFWD1; RNF119
• NCBI Protein Information: E3 ubiquitin-protein ligase TRAF7; RING finger protein 119; ring finger and WD repeat domain 1; RING finger and WD repeat-containing protein 1
• UniProt Protein Name: E3 ubiquitin-protein ligase TRAF7
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: TRAF7
• UniProt Synonym Gene Names: RFWD1; RNF119
• UniProt Entry Name: TRAF7_HUMAN

NCBI Description

Tumor necrosis factor (TNF; see MIM 191160) receptor-associated factors, such as TRAF7, are signal transducers for members of the TNF receptor superfamily (see MIM 191190). TRAFs are composed of an N-terminal cysteine/histidine-rich region containing zinc RING and/or zinc finger motifs; a coiled-coil (leucine zipper) motif; and a homologous region that defines the TRAF family, the TRAF domain, which is involved in self-association and receptor binding.[supplied by OMIM, Apr 2004]

Uniprot Description

TRAF7: E3 ubiquitin ligase capable of auto-ubiquitination, following phosphorylation by MAP3K3. Potentiates MEKK3-mediated activation of the NF-kappa-B, JUN/AP1 and DDIT3 transcriptional regulators. Induces apoptosis when overexpressed. Belongs to the WD repeat TRAF7 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; EC 6.3.2.-; Ubiquitin ligase; EC 6.3.2.19; Ligase

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: intracellular membrane-bound organelle; perinuclear region of cytoplasm; cytoplasmic membrane-bound vesicle; plasma membrane; nucleus; cytosol; ubiquitin ligase complex

Molecular Function: protein binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: positive regulation of protein sumoylation; regulation of protein localization; positive regulation of MAPKKK cascade; transcription, DNA-dependent; apoptosis; activation of MAPKKK activity; protein ubiquitination; negative regulation of transcription from RNA polymerase II promoter