TMED2 blocking peptide

TMED2 Antibody (N-term) Blocking Peptide

• Gene Names: TMED2; p24; P24A; RNP24; p24b1; p24beta1
• Synonyms: TMED2; TMED2 Antibody (N-term) Blocking Peptide; Transmembrane emp24 domain-containing protein 2; Membrane protein p24A; p24; p24 family protein beta-1; p24beta1; RNP24; TMED2 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of TMED2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 201

• Cellular Location: Cytoplasmic vesicle membrane; Single-pass type I membrane protein. Cytoplasmic vesicle, COPI-coated vesicle membrane; Single-pass type I membrane protein. Golgi apparatus, cis-Golgi network membrane; Single-pass type I membrane protein Golgi apparatus, Golgi stack membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type I membrane protein Note: Cycles between compartments of the early secretatory pathway
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for TMED2 blocking peptide

Involved in vesicular protein trafficking. Mainly functions in the early secretory pathway but also in post-Golgi membranes. Thought to act as cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and to be involved in vesicle coat formation at the cytoplasmic side. In COPII vesicle-mediated anterograde transport involved in the transport of GPI-anchored proteins and proposed to act together with TMED10 as their cargo receptor; the function specifically implies SEC24C and SEC24D of the COPII vesicle coat and lipid raft-like microdomains of the ER. Recognizes GPI anchors structural remodeled in the ER by PGAP1 and MPPE1. In COPI vesicle-mediated retrograde transport inhibits the GTPase- activating activity of ARFGAP1 towards ARF1 thus preventing immature uncoating and allowing cargo selection to take place. Involved in trafficking of G protein-coupled receptors (GPCRs). Regulates F2RL1, OPRM1 and P2RY4 exocytic trafficking from the Golgi to the plasma membrane thus contributing to receptor resensitization. Facilitates CASR maturation and stabilization in the early secretory pathway and increases CASR plasma membrane targeting. Proposed to be involved in organization of intracellular membranes such as the maintenance of the Golgi apparatus. May also play a role in the biosynthesis of secreted cargo such as eventual processing.

NCBI and Uniprot Product Information

• NCBI GI #: 3914237
• NCBI GeneID: 10959
• UniProt Accession #: Q15363
• Molecular Weight: 22,761 Da
• NCBI Official Full Name: Transmembrane emp24 domain-containing protein 2
• NCBI Official Synonym Full Names: transmembrane p24 trafficking protein 2
• NCBI Official Symbol: TMED2
• NCBI Official Synonym Symbols: p24; P24A; RNP24; p24b1; p24beta1
• NCBI Protein Information: transmembrane emp24 domain-containing protein 2
• UniProt Protein Name: Transmembrane emp24 domain-containing protein 2
• Protein Family: Transmembrane emp24 domain-containing protein
• UniProt Gene Name: TMED2
• UniProt Synonym Gene Names: RNP24; p24beta1
• UniProt Entry Name: TMED2_HUMAN

Uniprot Description

TMED2: Involved in vesicular protein trafficking. Mainly functions in the early secretory pathway but also in post-Golgi membranes. Thought to act as cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and to be involved in vesicle coat formation at the cytoplasmic side. In COPII vesicle-mediated anterograde transport involved in the transport of GPI-anchored proteins and proposed to act togther with TMED10 as their cargo receptor; the function specifically implies SEC24C and SEC24D of the COPII vesicle coat and lipid raft-like microdomains of the ER. Recognizes GPI anchors structural remodeled in the ER by PGAP1 and MPPE1. In COPI vesicle-mediated retrograde transport inhibits the GTPase- activating activity of ARFGAP1 towards ARF1 thus preventing immature uncoating and allowing cargo selection to take place. Involved in trafficking of G protein-coupled receptors (GPCRs). Regulates F2RL1, OPRM1 and P2RY4 exocytic trafficking from the Golgi to the plasma membrane thus contributing to receptor resensitization. Facilitates CASR maturation and stabilization in the early secretory pathway and increases CASR plasma membrane targeting. Proposed to be involved in organization of intracellular membranes such as the maintenance of the Golgi apparatus. May also play a role in the biosynthesis of secreted cargo such as eventual processing. Belongs to the EMP24/GP25L family.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: COPI-coated vesicle; endoplasmic reticulum; endoplasmic reticulum membrane; ER-Golgi intermediate compartment; ER-Golgi intermediate compartment membrane; Golgi apparatus; Golgi membrane; integral to membrane; intracellular membrane-bound organelle; transport vesicle; zymogen granule membrane

Molecular Function: protein binding

Biological Process: COPI coating of Golgi vesicle; COPII coating of Golgi vesicle; ER to Golgi vesicle-mediated transport; Golgi organization and biogenesis; intracellular protein transport; retrograde vesicle-mediated transport, Golgi to ER