SPATA18 blocking peptide

SPATA18 Antibody (N-term) Blocking peptide

• Gene Names: SPATA18; Mieap; SPETEX1
• Synonyms: SPATA18; SPATA18 Antibody (N-term) Blocking peptide; Mitochondria-eating protein; Spermatogenesis-associated protein 18; MIEAP; SPATA18 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of SPATA18. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 538

• Cellular Location: Cytoplasm. Mitochondrion outer membrane. Note: Localizes to the cytoplasm under normal conditions Relocalizes to mitochondrion outer membrane following cellular stress. Colocalizes with BNIP3 and BNIP3L at the mitochondrion outer membrane
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for SPATA18 blocking peptide

Key regulator of mitochondrial quality that mediates the repairing or degradation of unhealthy mitochondria in response to mitochondrial damage. Mediator of mitochondrial protein catabolic process (also named MALM) by mediating the degradation of damaged proteins inside mitochondria by promoting the accumulation in the mitochondrial matrix of hydrolases that are characteristic of the lysosomal lumen. Also involved in mitochondrion degradation of damaged mitochondria by promoting the formation of vacuole-like structures (named MIV), which engulf and degrade unhealthy mitochondria by accumulating lysosomes. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix.

NCBI and Uniprot Product Information

• NCBI GI #: 74751399
• NCBI GeneID: 132671
• UniProt Accession #: Q8TC71; Q8IY48; Q8N7D7; B4E2R0; E5RLK1
• Molecular Weight: 57,485 Da
• NCBI Official Full Name: Mitochondria-eating protein
• NCBI Official Synonym Full Names: spermatogenesis associated 18
• NCBI Official Symbol: SPATA18
• NCBI Official Synonym Symbols: Mieap; SPETEX1
• NCBI Protein Information: mitochondria-eating protein
• UniProt Protein Name: Mitochondria-eating protein
• Protein Family: Mitochondria-eating protein
• UniProt Gene Name: SPATA18
• UniProt Synonym Gene Names: MIEAP
• UniProt Entry Name: MIEAP_HUMAN

Uniprot Description

SPATA18: Key regulator of mitochondrial quality that mediates the repairing or degradation of unhealthy mitochondria in response to mitochondrial damage. Mediator of mitochondrial protein catabolic process (also named MALM) by mediating the degradation of damaged proteins inside mitochondria by promoting the accumulation in the mitochondrial matrix of hydrolases that are characteristic of the lysosomal lumen. Also involved in mitochondrion degradation of damaged mitochondria by promoting the formation of vacuole-like structures (named MIV), which engulf and degrade unhealthy mitochondria by accumulating lysosomes. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. Belongs to the MIEAP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 4q12

Cellular Component: cytoplasm; intracellular membrane-bound organelle; mitochondrial outer membrane

Molecular Function: protein binding

Biological Process: response to DNA damage stimulus