SOCS1 blocking peptide

SOCS1 Antibody (N-term) Blocking Peptide

• Gene Names: SOCS1; JAB; CIS1; SSI1; TIP3; CISH1; SSI-1; SOCS-1
• Synonyms: SOCS1; SOCS1 Antibody (N-term) Blocking Peptide; Suppressor of cytokine signaling 1; SOCS-1; JAK-binding protein; JAB; STAT-induced STAT inhibitor 1; SSI-1; Tec-interacting protein 3; TIP-3; SSI1; TIP3; SOCS1 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of human SOCS1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 211

• Cellular Location: Nucleus. Cytoplasmic vesicle. Note: Detected in perinuclear cytoplasmic vesicles upon interaction with FGFR3
• Tissue Location: Expressed in all tissues with high expression in spleen, small intestine and peripheral blood leukocytes
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for SOCS1 blocking peptide

SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein- tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival (By similarity). Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2. SOCS1 appears to be a negative regulator in IGF1R signaling pathway.

NCBI and Uniprot Product Information

• NCBI GI #: 20178097
• NCBI GeneID: 8651
• UniProt Accession #: O15524; O15097; Q9NSA7
• Molecular Weight: 23,551 Da
• NCBI Official Full Name: Suppressor of cytokine signaling 1
• NCBI Official Synonym Full Names: suppressor of cytokine signaling 1
• NCBI Official Symbol: SOCS1
• NCBI Official Synonym Symbols: JAB; CIS1; SSI1; TIP3; CISH1; SSI-1; SOCS-1
• NCBI Protein Information: suppressor of cytokine signaling 1
• UniProt Protein Name: Suppressor of cytokine signaling 1
• Protein Family: Suppressor of cytokine signaling
• UniProt Gene Name: SOCS1
• UniProt Synonym Gene Names: SSI1; TIP3; SOCS-1; JAB; SSI-1; TIP-3
• UniProt Entry Name: SOCS1_HUMAN

NCBI Description

This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by a subset of cytokines, including IL2, IL3 erythropoietin (EPO), CSF2/GM-CSF, and interferon (IFN)-gamma. The protein encoded by this gene functions downstream of cytokine receptors, and takes part in a negative feedback loop to attenuate cytokine signaling. Knockout studies in mice suggested the role of this gene as a modulator of IFN-gamma action, which is required for normal postnatal growth and survival. [provided by RefSeq, Jul 2008]

Uniprot Description

SOCS1: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein- tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival. Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2. SOCS1 appears to be a negative regulator in IGF1R signaling pathway. Interacts with multiple activated signaling proteins of the tyrosine kinase signaling pathway including JAK family kinases, TEC, KIT, GRB2 and VAV. Binding to JAKs is mediated through the KIR and SH2 domains to a phosphorylated tyrosine residue within the JAK JH1 domain. Binds the SH3 domain of GRB2 via diproline determinants in the N-terminus, and the N-terminal regulatory domain of VAV. Interacts with the Elongin BC complex (TCEB1 and TCEB2). Component of an ECS CBC(SOCS1) E3 ubiquitin-protein ligase complex which contains Elongin BC, CUL5, RBX1 and SOCS1. Interacts (via SH2 domain and SOCS box) with TRIM8. Interacts with AXL, CUL2 and FGFR3. Interacts with INSR. By a subset of cytokines including those belonging to the interferon, interleukin and colony-stimulating factor families. Expressed in all tissues with high expression in spleen, small intestine and peripheral blood leukocytes.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 16p13.13

Cellular Component: cytoplasm; cytosol

Molecular Function: insulin-like growth factor receptor binding; kinase inhibitor activity; protein binding; protein kinase binding; protein kinase inhibitor activity

Biological Process: cytokine and chemokine mediated signaling pathway; negative regulation of insulin receptor signaling pathway; negative regulation of JAK-STAT cascade; negative regulation of tyrosine phosphorylation of Stat3 protein; regulation of protein amino acid phosphorylation