SMC3 blocking peptide

SMC3 Antibody (Center) Blocking Peptide

• Gene Names: SMC3; BAM; BMH; HCAP; CDLS3; CSPG6; SMC3L1
• Synonyms: SMC3; SMC3 Antibody (Center) Blocking Peptide; Structural maintenance of chromosomes protein 3; SMC protein 3; SMC-3; Basement membrane-associated chondroitin proteoglycan; Bamacan; Chondroitin sulfate proteoglycan 6; Chromosome-associated polypeptide; hCAP; BAM; BMH; CSPG6; SMC3L1; SMC3 blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 1217

• Cellular Location: Nucleus. Chromosome. Chromosome, centromere. Note: Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. The phosphorylated form at Ser- 1083 is preferentially associated with unsynapsed chromosomal regions (By similarity).
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for SMC3 blocking peptide

Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement.

NCBI and Uniprot Product Information

• NCBI GI #: 29337005
• NCBI GeneID: 9126
• UniProt Accession #: Q9UQE7; O60464; Q5T482; A8K156
• Molecular Weight: 141,542 Da
• NCBI Official Full Name: Structural maintenance of chromosomes protein 3
• NCBI Official Synonym Full Names: structural maintenance of chromosomes 3
• NCBI Official Symbol: SMC3
• NCBI Official Synonym Symbols: BAM; BMH; HCAP; CDLS3; CSPG6; SMC3L1
• NCBI Protein Information: structural maintenance of chromosomes protein 3
• UniProt Protein Name: Structural maintenance of chromosomes protein 3
• UniProt Gene Name: SMC3
• UniProt Synonym Gene Names: BAM; BMH; CSPG6; SMC3L1; SMC protein 3; SMC-3; Bamacan; hCAP
• UniProt Entry Name: SMC3_HUMAN

NCBI Description

This gene belongs to the SMC3 subfamily of SMC proteins. The encoded protein occurs in certain cell types as either an intracellular, nuclear protein or a secreted protein. The nuclear form, known as structural maintenance of chromosomes 3, is a component of the multimeric cohesin complex that holds together sister chromatids during mitosis, enabling proper chromosome segregation. Post-translational modification of the encoded protein by the addition of chondroitin sulfate chains gives rise to the secreted proteoglycan bamacan, an abundant basement membrane protein. [provided by RefSeq, Jul 2008]

Uniprot Description

SMC3: Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement. Interacts with MXI1, MXD3 and MXD4. Interacts with SYCP2. Found in a complex with SMC1A, CDCA5 and RAD21, PDS5A/APRIN and PDS5B/SCC-112. Forms a heterodimer with SMC1A or SMC1B in cohesin complexes. Cohesin complexes are composed of the SMC1 (SMC1A or SMC1B) and SMC3 heterodimer attached via their hinge domain, RAD21 which link them, and one STAG protein (STAG1, STAG2 or STAG3), which interacts with RAD21. Also found in meiosis-specific cohesin complexes. Interacts with NUMA1, and forms a ternary complex with KIF3B and KIFAP3, suggesting a function in tethering the chromosomes to the spindle pole and in chromosome movement. Interacts with PDS5A and WAPAL; regulated by SMC3 acetylation. Belongs to the SMC family. SMC3 subfamily.

Protein type: DNA repair, damage; Cell cycle regulation

Chromosomal Location of Human Ortholog: 10q25

Cellular Component: chromatin; chromosome; chromosome, pericentric region; cytoplasm; cytosol; nuclear matrix; nucleoplasm; nucleus; spindle pole

Molecular Function: dynein binding; protein binding; protein heterodimerization activity

Biological Process: meiosis; mitosis; mitotic spindle organization and biogenesis; negative regulation of DNA endoreduplication; protein sumoylation; regulation of DNA replication; signal transduction; sister chromatid cohesion

Disease: Cornelia De Lange Syndrome 3