SMC1B blocking peptide

SMC1B Antibody (Center) Blocking peptide

• Gene Names: SMC1B; SMC1L2; SMC1BETA
• Synonyms: SMC1B; SMC1B Antibody (Center) Blocking peptide; Structural maintenance of chromosomes protein 1B; SMC protein 1B; SMC-1-beta; SMC-1B; SMC1L2; SMC1B blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the Center region of SMC1B. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 1235

• Cellular Location: Nucleus. Chromosome. Chromosome, centromere. Note: Associates with chromatin. In prophase I stage of meiosis, localizes along the AE of synaptonemal complexes. In late-pachytene-diplotene, the bulk of protein dissociates from the chromosome arms probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. Remains chromatin associated at the centromeres up to metaphase II. At anaphase II, dissociates from centromeres, allowing chromosomes segregation (By similarity).
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for SMC1B blocking peptide

Meiosis-specific component of cohesin complex. Required for the maintenance of meiotic cohesion, but not, or only to a minor extent, for its establishment. Contributes to axial element (AE) formation and the organization of chromatin loops along the AE. Plays a key role in synapsis, recombination and chromosome movements. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The meiosis-specific cohesin complex probably replaces mitosis specific cohesin complex when it dissociates from chromatin during prophase I (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 57015410
• NCBI GeneID: 27127
• UniProt Accession #: Q8NDV3; Q5TIC3; Q6ZUF9; Q9Y3G5; A0AV46; B0QY23; B0QY24
• Molecular Weight: 143,837 Da
• NCBI Official Full Name: Structural maintenance of chromosomes protein 1B
• NCBI Official Synonym Full Names: structural maintenance of chromosomes 1B
• NCBI Official Symbol: SMC1B
• NCBI Official Synonym Symbols: SMC1L2; SMC1BETA
• NCBI Protein Information: structural maintenance of chromosomes protein 1B
• UniProt Protein Name: Structural maintenance of chromosomes protein 1B
• Protein Family: Structural maintenance of chromosomes protein
• UniProt Gene Name: SMC1B
• UniProt Synonym Gene Names: SMC1L2; SMC protein 1B; SMC-1-beta; SMC-1B
• UniProt Entry Name: SMC1B_HUMAN

NCBI Description

SMC1L2 belongs to a family of proteins required for chromatid cohesion and DNA recombination during meiosis and mitosis (3:Revenkova et al., 2001 [PubMed 11564881]).[supplied by OMIM, Mar 2008]

Uniprot Description

SMC1B: Meiosis-specific component of cohesin complex. Required for the maintenance of meiotic cohesion, but not, or only to a minor extent, for its establishment. Contributes to axial element (AE) formation and the organization of chromatin loops along the AE. Plays a key role in synapsis, recombination and chromosome movements. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The meiosis-specific cohesin complex probably replaces mitosis specific cohesin complex when it dissociates from chromatin during prophase I. Belongs to the SMC family. SMC1 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 22q13.31

Cellular Component: cytoplasm; nucleoplasm