SMAD4 (T277) Blocking Peptide | SMAD4 blocking peptide

Phospho-SMAD4(T277) Antibody Blocking peptide

• Gene Names: SMAD4; JIP; DPC4; MADH4; MYHRS
• Synonyms: SMAD4 (T277); Phospho-SMAD4(T277) Antibody Blocking peptide; Mothers against decapentaplegic homolog 4; MAD homolog 4; Mothers against DPP homolog 4; Deletion target in pancreatic carcinoma 4; SMAD family member 4; SMAD 4; Smad4; hSMAD4; SMAD4; DPC4; MADH4; SMAD4 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the center region of human SMAD4-T277. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 552

• Cellular Location: Cytoplasm. Nucleus. Note: Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with R- SMAD. PDPK1 prevents its nuclear translocation in response to TGF- beta
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for SMAD4 blocking peptide

In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (By similarity). Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

NCBI and Uniprot Product Information

• NCBI GI #: 13959561
• NCBI GeneID: 4089
• UniProt Accession #: Q13485; A8K405
• Molecular Weight: 60,439 Da
• NCBI Official Full Name: Mothers against decapentaplegic homolog 4
• NCBI Official Synonym Full Names: SMAD family member 4
• NCBI Official Symbol: SMAD4
• NCBI Official Synonym Symbols: JIP; DPC4; MADH4; MYHRS
• NCBI Protein Information: mothers against decapentaplegic homolog 4
• UniProt Protein Name: Mothers against decapentaplegic homolog 4
• Protein Family: Mothers against decapentaplegic
• UniProt Gene Name: SMAD4
• UniProt Synonym Gene Names: DPC4; MADH4; MAD homolog 4; Mothers against DPP homolog 4; SMAD 4; Smad4; hSMAD4
• UniProt Entry Name: SMAD4_HUMAN

NCBI Description

This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. [provided by RefSeq, Oct 2009]

Uniprot Description

SMAD4: transcription factor that mediates signal transduction by the transforming growth factor superfamily. The common smad (co-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. Promotes binding of the Smad2/Smad4/Fast-1 complex to DNA and provides an activation function required for Smad1 or Smad2 to stimulate transcription.

Protein type: Nuclear receptor co-regulator; DNA-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 18q21.1

Cellular Component: cytoplasm; cytosol; nuclear chromatin; nucleoplasm; nucleus; transcription factor complex

Molecular Function: DNA binding; identical protein binding; protein binding; protein homodimerization activity; sequence-specific DNA binding; transcription factor activity; transforming growth factor beta receptor, common-partner cytoplasmic mediator activity

Biological Process: BMP signaling pathway; cellular iron ion homeostasis; negative regulation of cell growth; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; palate development; positive regulation of BMP signaling pathway; positive regulation of histone H3-K4 methylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of transforming growth factor beta receptor signaling pathway; regulation of transforming growth factor beta receptor signaling pathway; regulation of transforming growth factor-beta2 production; response to hypoxia; SMAD protein complex assembly; somatic stem cell maintenance; transforming growth factor beta receptor signaling pathway

Disease: Juvenile Polyposis Syndrome; Juvenile Polyposis/hereditary Hemorrhagic Telangiectasia Syndrome; Myhre Syndrome; Pancreatic Cancer