SLC9A3R2 blocking peptide

SLC9A3R2 Blocking Peptide

Cat. Num. AAA9624373

• Gene Names: SLC9A3R2; SIP1; OCTS2; SIP-1; TKA-1; E3KARP; NHERF2; NHE3RF2; NHERF-2
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: SLC9A3R2; SLC9A3R2 Blocking Peptide; E3KARP; HGNC:11076; Isoform 3 regulator 2; Isoform 3 regulatory factor 2; MGC104639; Na(+)/H(+) exchange regulatory cofactor NHE RF2; Na(+)/H(+) exchange regulatory cofactor NHE-RF2; NHE3 kinase A regulatory protein; NHE3 kinase A regulatory protein E3KARP; NHE3 regulatory factor 2; NHE3RF2; NHERF 2; NHERF-2; NHERF2; NHRF2_HUMAN; OCTS2; SIP 1; SIP-1; SIP1; Slc9a3r2; Sodium hydrogen exchanger regulatory factor 2; Sodium-hydrogen exchanger regulatory factor 2; Sodium/hydrogen exchanger; Sodium/hydrogen exchanger regulatory factor 2; Solute carrier family 9 (sodium/hydrogen exchanger); Solute carrier family 9 (sodium/hydrogen exchanger) isoform 3 regulator 2; Solute carrier family 9 (sodium/hydrogen exchanger) isoform 3 regulatory factor 2; Solute carrier family 9 (sodium/hydrogen exchanger) member 3 regulator 2; Solute carrier family 9 isoform 3 regulator 2; Solute carrier family 9 isoform A3 regulatory factor 2; SRY interacting protein 1; SRY-interacting protein 1; TKA 1; TKA-1; TKA1; Tyrosine kinase activator protein 1; SLC9A3R2 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for SLC9A3R2 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for SLC9A3R2 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 194018553
• NCBI GeneID: 9351
• NCBI Accession #: NP_001123484.1
• NCBI GenBank Nucleotide #: NM_001130012.2
• UniProt Accession #: Q15599; O00272; O00556; Q3KQY7; D3DU84; D3DU85; H3BSV6
• Molecular Weight: 37,414 Da
• NCBI Official Full Name: Na(+)/H(+) exchange regulatory cofactor NHE-RF2 isoform a
• NCBI Official Synonym Full Names: solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 2
• NCBI Official Symbol: SLC9A3R2
• NCBI Official Synonym Symbols: SIP1; OCTS2; SIP-1; TKA-1; E3KARP; NHERF2; NHE3RF2; NHERF-2
• NCBI Protein Information: Na(+)/H(+) exchange regulatory cofactor NHE-RF2; Na(+)/H(+) exchange regulatory cofactor NHE-RF2; NHE3 regulatory factor 2; SRY-interacting protein 1; tyrosine kinase activator protein 1; NHE3 kinase A regulatory protein E3KARP; solute carrier family 9 is
• UniProt Protein Name: Na(+)/H(+) exchange regulatory cofactor NHE-RF2
• Protein Family: Na(+)/H(+) exchange regulatory cofactor
• UniProt Gene Name: SLC9A3R2
• UniProt Synonym Gene Names: NHERF2; NHERF-2; SIP-1; TKA-1
• UniProt Entry Name: NHRF2_HUMAN

NCBI Description

This gene encodes a member of the NHERF family of PDZ scaffolding proteins. These proteins mediate many cellular processes by binding to and regulating the membrane expression and protein-protein interactions of membrane receptors and transport proteins. The encoded protein plays a role in intestinal sodium absorption by regulating the activity of the sodium/hydrogen exchanger 3, and may also regulate the cystic fibrosis transmembrane regulator (CFTR) ion channel. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

NHERF-2: Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May also act as scaffold protein in the nucleus. Homodimer, and heterodimer with SLC9A3R1. Binds PDZK1. Found in a complex with EZR, PODXL and SLC9A3R2. Interacts (via the PDZ domains) with PODXL (via the C-terminal PDZ-binding motif DTHL); interaction is detected in glomerular epithelium cells. Binds ADRB2, SLC9A3, P2RY1, P2YR2, SRY, RDX and LPAR2. Interacts with MCC and PODXL. Interacts with SGK1 and KCNJ1/ROMK1. Widely expressed. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: focal adhesion; cytoplasm; apical plasma membrane; plasma membrane; endomembrane system; nucleus

Molecular Function: protein C-terminus binding; protein binding; beta-catenin binding; receptor binding; phosphatase binding

Biological Process: protein complex assembly; negative regulation of phosphoinositide 3-kinase cascade