SAE2 (UBA2) Blocking Peptide | UBA2 blocking peptide

SAE2 (UBA2) Antibody (C-term E616) Blocking peptide

• Gene Names: UBA2; ARX; SAE2; HRIHFB2115
• Synonyms: SAE2 (UBA2); SAE2 (UBA2) Antibody (C-term E616) Blocking peptide; SUMO-activating enzyme subunit 2; 632-; Anthracycline-associated resistance ARX; Ubiquitin-like 1-activating enzyme E1B; Ubiquitin-like modifier-activating enzyme 2; UBA2; SAE2; UBLE1B; UBA2 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human UBA2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 640

• Cellular Location: Cytoplasm. Nucleus. Note: Shuttles between the cytoplasm and the nucleus, sumoylation is required either for nuclear translocation or nuclear retention
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for UBA2 blocking peptide

The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.

NCBI and Uniprot Product Information

• NCBI GI #: 42559898
• NCBI GeneID: 10054
• UniProt Accession #: Q9UBT2; O95605; Q59H87; Q6IBP6; Q9NTJ1; Q9UED2; B3KWB9
• Molecular Weight: 60,839 Da
• NCBI Official Full Name: SUMO-activating enzyme subunit 2
• NCBI Official Synonym Full Names: ubiquitin like modifier activating enzyme 2
• NCBI Official Symbol: UBA2
• NCBI Official Synonym Symbols: ARX; SAE2; HRIHFB2115
• NCBI Protein Information: SUMO-activating enzyme subunit 2
• UniProt Protein Name: SUMO-activating enzyme subunit 2
• Protein Family: SUMO-activating enzyme
• UniProt Gene Name: UBA2
• UniProt Synonym Gene Names: SAE2; UBLE1B
• UniProt Entry Name: SAE2_HUMAN

NCBI Description

Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 (MIM 613294) and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010]

Uniprot Description

SAE2: a protein of the ubiquitin-activating E1 family. Acts as a UBL1 E1 ligase. Mediates ATP-dependent activation of UBL1 and formation of a thiolester with a conserved cysteine residue on SAE2.

Protein type: EC 6.3.2.19; Motility/polarity/chemotaxis; Ubiquitin conjugating system; EC 6.3.2.-; Ubiquitin ligase; Ligase

Chromosomal Location of Human Ortholog: 19q12

Cellular Component: cytosol; nucleoplasm

Molecular Function: enzyme activator activity; protein binding; protein heterodimerization activity; SUMO activating enzyme activity

Biological Process: protein sumoylation