RPA2) Blocking Peptide | RPA2 blocking peptide

RPA2 Antibody (N-term)) Blocking Peptide

• Gene Names: RPA2; REPA2; RPA32; RP-A p32; RP-A p34
• Synonyms: RPA2); RPA2 Antibody (N-term)) Blocking Peptide; Replication protein A 32 kDa subunit; RP-A p32; Replication factor A protein 2; RF-A protein 2; Replication protein A 34 kDa subunit; RP-A p34; RPA2; REPA2; RPA32; RPA34; RPA2 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of human RPA2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 270

• Cellular Location: Nucleus. Nucleus, PML body. Note: Redistributes to discrete nuclear foci upon DNA damage in an ATR-dependent manner
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for RPA2 blocking peptide

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.

NCBI and Uniprot Product Information

• NCBI GI #: 132474
• NCBI GeneID: 6118
• UniProt Accession #: P15927; Q52II0; Q5TEI9; Q5TEJ5
• Molecular Weight: 38,810 Da
• NCBI Official Full Name: Replication protein A 32 kDa subunit
• NCBI Official Synonym Full Names: replication protein A2
• NCBI Official Symbol: RPA2
• NCBI Official Synonym Symbols: REPA2; RPA32; RP-A p32; RP-A p34
• NCBI Protein Information: replication protein A 32 kDa subunit
• UniProt Protein Name: Replication protein A 32 kDa subunit
• Protein Family: Replication protein
• UniProt Gene Name: RPA2
• UniProt Synonym Gene Names: REPA2; RPA32; RPA34; RP-A p32; RF-A protein 2; RP-A p34
• UniProt Entry Name: RFA2_HUMAN

Uniprot Description

RFA2: Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage. Heterotrimer of 70, 32 and 14 kDa chains (canonical replication protein A complex). Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN. Directly interacts with PPP4R2, but not with SMEK2; this interaction is DNA damage-dependent and leads RPA2 dephosphorylation by PPP4C recruitment. Interacts with RAD51, preferentially when hyperphosphorylated. Directly interacts with RFWD3. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA replication

Chromosomal Location of Human Ortholog: 1p35

Cellular Component: chromatin; chromosome, telomeric region; DNA replication factor A complex; nuclear chromosome, telomeric region; nucleoplasm; nucleus; PML body

Molecular Function: damaged DNA binding; enzyme binding; protein binding; protein phosphatase binding; single-stranded DNA binding; ubiquitin protein ligase binding

Biological Process: base-excision repair; bypass DNA synthesis; DNA damage response, detection of DNA damage; DNA replication; double-strand break repair via homologous recombination; error-prone postreplication DNA repair; G1 DNA damage checkpoint; G1/S transition of mitotic cell cycle; mismatch repair; nucleotide-excision repair; nucleotide-excision repair, DNA gap filling; nucleotide-excision repair, DNA incision; nucleotide-excision repair, DNA incision, 3'-to lesion; nucleotide-excision repair, DNA incision, 5'-to lesion; nucleotide-excision repair, preincision complex assembly; nucleotide-excision repair, preincision complex stabilization; telomere maintenance; telomere maintenance via recombination; transcription-coupled nucleotide-excision repair