RHOH blocking peptide

RHOH Antibody (C-term) Blocking Peptide

• Gene Names: RHOH; TTF; ARHH
• Synonyms: RHOH; RHOH Antibody (C-term) Blocking Peptide; Rho-related GTP-binding protein RhoH; GTP-binding protein TTF; Translocation three four protein; ARHH; TTF; RHOH blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 191

• Cellular Location: Cytoplasm. Cell membrane; Lipid- anchor; Cytoplasmic side Note: Colocalizes together with ZAP70 in the immunological synapse
• Tissue Location: Expressed only in hematopoietic cells. Present at very high levels in the thymus, less abundant in the spleen, and least abundant in the bone marrow. Expressed at a higher level in the TH1 subtype of T-helper cells than in the TH2 subpopulation. Expressed in neutrophils under inflammatory conditions, such as cystic fibrosis, ulcerative colitis and appendicitis.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for RHOH blocking peptide

Negative regulator of hematopoietic progenitor cell proliferation, survival and migration. Critical regulator of thymocyte development and T-cell antigen receptor (TCR) signaling by mediating recruitment and activation of ZAP70. Required for phosphorylation of CD3Z, membrane translocation of ZAP70 and subsequent activation of the ZAP70-mediated pathways. Essential for efficient beta-selection and positive selection by promoting the ZAP70-dependent phosphorylation of the LAT signalosome during pre-TCR and TCR signaling. Crucial for thymocyte maturation during DN3 to DN4 transition and during positive selection. Plays critical roles in mast cell function by facilitating phosphorylation of SYK in Fc epsilon RI-mediated signal transduction. Essential for the phosphorylation of LAT, LCP2, PLCG1 and PLCG2 and for Ca(2+) mobilization in mast cells (By similarity). Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins. Inhibits the activation of NF-kappa-B by TNF and IKKB and the activation of CRK/p38 by TNF. Inhibits activities of RAC1, RHOA and CDC42. Negatively regulates leukotriene production in neutrophils.

NCBI and Uniprot Product Information

• NCBI GI #: 2500200
• NCBI GeneID: 399
• UniProt Accession #: Q15669
• Molecular Weight: 21,331 Da
• NCBI Official Full Name: Rho-related GTP-binding protein RhoH
• NCBI Official Synonym Full Names: ras homolog family member H
• NCBI Official Symbol: RHOH
• NCBI Official Synonym Symbols: TTF; ARHH
• NCBI Protein Information: rho-related GTP-binding protein RhoH
• UniProt Protein Name: Rho-related GTP-binding protein RhoH
• Protein Family: Rho-related GTP-binding protein
• UniProt Gene Name: RHOH
• UniProt Synonym Gene Names: ARHH; TTF
• UniProt Entry Name: RHOH_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Ras superfamily of guanosine triphosphate (GTP)-metabolizing enzymes. The encoded protein is expressed in hematopoietic cells, where it functions as a negative regulator of cell growth and survival. This gene may be hypermutated or misexpressed in leukemias and lymphomas. Chromosomal translocations in non-Hodgkin's lymphoma occur between this locus and B-cell CLL/lymphoma 6 (BCL6) on chromosome 3, leading to the production of fusion transcripts. Alternative splicing in the 5' untranslated region results in multiple transcript variants that encode the same protein. [provided by RefSeq, May 2013]

Uniprot Description

RHOH: Negative regulator of hematopoietic progenitor cell proliferation, survival and migration. Critical regulator of thymocyte development and T-cell antigen receptor (TCR) signaling by mediating recruitment and activation of ZAP70. Required for phosphorylation of CD3Z, membrane translocation of ZAP70 and subsequent activation of the ZAP70-mediated pathways. Essential for efficient beta-selection and positive selection by promoting the ZAP70-dependent phosphorylation of the LAT signalosome during pre-TCR and TCR signaling. Crucial for thymocyte maturation during DN3 to DN4 transition and during positive selection. Plays critical roles in mast cell function by facilitating phosphorylation of SYK in Fc epsilon RI-mediated signal transduction. Essential for the phosphorylation of LAT, LCP2, PLCG1 and PLCG2 and for Ca(2+) mobilization in mast cells. Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins. Inhibits the activation of NF-kappa-B by TNF and IKKB and the activation of CRK/p38 by TNF. Inhibits activities of RAC1, RHOA and CDC42. Negatively regulates leukotriene production in neutrophils. A chromosomal aberration involving RHOH is found in a non-Hodgkin lymphoma cell line. Translocation t(3;4)(q27;p11) with BCL6. Belongs to the small GTPase superfamily. Rho family.

Protein type: G protein, monomeric, Rho; G protein, monomeric; G protein

Chromosomal Location of Human Ortholog: 4p13

Cellular Component: cytoplasm; cytosol; plasma membrane

Molecular Function: GTP binding; kinase inhibitor activity; protein binding

Biological Process: negative regulation of I-kappaB kinase/NF-kappaB cascade; regulation of small GTPase mediated signal transduction