RBBP8 blocking peptide

RBBP8 Antibody (C-term) Blocking peptide

• Gene Names: RBBP8; RIM; COM1; CTIP; JWDS; SAE2; SCKL2
• Synonyms: RBBP8; RBBP8 Antibody (C-term) Blocking peptide; DNA endonuclease RBBP8; 31--; CtBP-interacting protein; CtIP; Retinoblastoma-binding protein 8; RBBP-8; Retinoblastoma-interacting protein and myosin-like; RIM; Sporulation in the absence of SPO11 protein 2 homolog; SAE2; CTIP; RBBP8 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of RBBP8. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 897

• Cellular Location: Nucleus. Note: Associates with sites of DNA damage in S/G2 phase. Ubiquitinated RBBP8 binds to chromatin following DNA damage
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for RBBP8 blocking peptide

Endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in processing meiotic and mitotic double-strand breaks (DSBs) by ensuring both resection and intrachromosomal association of the broken ends. Functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA. Component of the BRCA1-RBBP8 complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage. Promotes microhomology-mediated alternative end joining (A-NHEJ) during class-switch recombination and plays an essential role in chromosomal translocations.

NCBI and Uniprot Product Information

• NCBI GI #: 116242745
• NCBI GeneID: 5932
• UniProt Accession #: Q99708; O75371; Q8NHQ3; A6NKN2; A8K8W6; E7ETY1
• Molecular Weight: 98,434 Da
• NCBI Official Full Name: DNA endonuclease RBBP8
• NCBI Official Synonym Full Names: RB binding protein 8, endonuclease
• NCBI Official Symbol: RBBP8
• NCBI Official Synonym Symbols: RIM; COM1; CTIP; JWDS; SAE2; SCKL2
• NCBI Protein Information: DNA endonuclease RBBP8
• UniProt Protein Name: DNA endonuclease RBBP8
• Protein Family: RBBP8 N-terminal-like protein
• UniProt Gene Name: RBBP8
• UniProt Synonym Gene Names: CTIP; CtIP; RBBP-8; RIM; SAE2
• UniProt Entry Name: COM1_HUMAN

NCBI Description

The protein encoded by this gene is a ubiquitously expressed nuclear protein. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. This protein complexes with transcriptional co-repressor CTBP. It is also associated with BRCA1 and is thought to modulate the functions of BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. It is suggested that this gene may itself be a tumor suppressor acting in the same pathway as BRCA1. Three transcript variants encoding two different isoforms have been found for this gene. More transcript variants exist, but their full-length natures have not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

CtIP: a transcription factor containing C2H2 zinc fingers. May modulate the functions ascribed to BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. Interacts with CTBP, with the C-terminal (BRCT) domains of BRCA1, and with the retinoblastoma protein. Abundantly expressed in brain and the immune system and associated with immune system malignancies. Ionizing radiation induces its hyperphosphorylation and dissociation from BRCA1 in an ATM-dependent manner.

Protein type: Transcription, coactivator/corepressor; EC 3.1.-.-

Chromosomal Location of Human Ortholog: 18q11.2

Cellular Component: nucleolus; nucleoplasm; nucleus; transcriptional repressor complex

Molecular Function: damaged DNA binding; protein binding; single-stranded DNA specific endodeoxyribonuclease activity

Biological Process: cell cycle checkpoint; DNA double-strand break processing; DNA repair; DNA replication; DNA synthesis during DNA repair; double-strand break repair via homologous recombination; G2/M transition DNA damage checkpoint; nucleotide-excision repair; regulation of transcription from RNA polymerase II promoter; strand displacement

Disease: Jawad Syndrome; Seckel Syndrome 2