RARA blocking peptide

RARA Antibody (C-term) Blocking peptide

• Gene Names: RARA; RAR; NR1B1
• Synonyms: RARA; RARA Antibody (C-term) Blocking peptide; Retinoic acid receptor alpha; RAR-alpha; Nuclear receptor subfamily 1 group B member 1; NR1B1; RARA blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of RARA. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 462

• Cellular Location: Nucleus. Cytoplasm. Note: Nuclear localization depends on ligand binding, phosphorylation and sumoylation. Transloaction to the nucleus in the absence of ligand is dependent on activation of PKC and the downstream MAPK phosphorylation
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for RARA blocking peptide

Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. RARA plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis. Has a role in the survival of early spermatocytes at the beginning prophase of meiosis. In Sertoli cells, may promote the survival and development of early meiotic prophase spermatocytes. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function (By similarity). Regulates expression of target genes in a ligand- dependent manner by recruiting chromatin complexes containing KMT2E/MLL5. Mediates retinoic acid-induced granulopoiesis.

NCBI and Uniprot Product Information

• NCBI GI #: 133483
• NCBI GeneID: 5914
• UniProt Accession #: P10276; P78456; Q13440; Q13441; Q96S41; Q9NQS0; B8Y636
• Molecular Weight: 39,700 Da
• NCBI Official Full Name: Retinoic acid receptor alpha
• NCBI Official Synonym Full Names: retinoic acid receptor alpha
• NCBI Official Symbol: RARA
• NCBI Official Synonym Symbols: RAR; NR1B1
• NCBI Protein Information: retinoic acid receptor alpha
• UniProt Protein Name: Retinoic acid receptor alpha
• Protein Family: Retinoic acid receptor
• UniProt Gene Name: RARA
• UniProt Synonym Gene Names: NR1B1; RAR-alpha
• UniProt Entry Name: RARA_HUMAN

NCBI Description

This gene represents a nuclear retinoic acid receptor. The encoded protein, retinoic acid receptor alpha, regulates transcription in a ligand-dependent manner. This gene has been implicated in regulation of development, differentiation, apoptosis, granulopoeisis, and transcription of clock genes. Translocations between this locus and several other loci have been associated with acute promyelocytic leukemia. Alternatively spliced transcript variants have been found for this locus.[provided by RefSeq, Sep 2010]

Uniprot Description

RARA: is a receptor for retinoic acid, a potent mammalian morphogen and teratogen that has profound effects on vertebrate development. RARA is a member of the nuclear receptor superfamily. Controls cell function by directly regulating gene expression. Its phosphorylation is crucial for transcriptional activity. Aberrations involving RARA may be a cause of acute promyelocytic leukemia. Two splice-variant isoforms have been described.

Protein type: Oncoprotein; DNA-binding; Nuclear receptor; Transcription factor

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: actin cytoskeleton; cell surface; cytoplasm; nuclear chromatin; nucleoplasm; nucleus

Molecular Function: alpha-actinin binding; chromatin DNA binding; enzyme binding; protein binding; protein domain specific binding; protein heterodimerization activity; protein kinase A binding; protein kinase B binding; receptor binding; retinoic acid binding; retinoic acid receptor activity; transcription coactivator activity; transcription corepressor activity; transcription factor activity; transcription factor binding

Biological Process: apoptotic cell clearance; negative regulation of granulocyte differentiation; negative regulation of interferon-gamma production; negative regulation of transcription, DNA-dependent; negative regulation of tumor necrosis factor production; positive regulation of binding; positive regulation of cell cycle; positive regulation of cell proliferation; positive regulation of interleukin-13 production; positive regulation of interleukin-4 production; positive regulation of interleukin-5 production; positive regulation of T-helper 2 cell differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation; response to retinoic acid; retinoic acid receptor signaling pathway; signal transduction; transcription initiation from RNA polymerase II promoter

Disease: Acute Promyelocytic Leukemia