PUM2 (S182) Blocking Peptide | PUM2 blocking peptide

PUM2 Antibody (S182) Blocking Peptide

• Gene Names: PUM2; PUMH2; PUML2
• Synonyms: PUM2 (S182); PUM2 Antibody (S182) Blocking Peptide; Pumilio homolog 2; Pumilio-2; PUM2; KIAA0235; PUMH2; PUM2 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the S182 region of human PUM2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 1066

• Cellular Location: Cytoplasm. Cytoplasmic granule. Cytoplasm, perinuclear region. Note: The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). Colocalizes with NANOS1 and SNAPIN in the perinuclear region of germ cells.
• Tissue Location: Expressed in male germ cells of adult testis (at protein level). Highly expressed in testis and ovary Predominantly expressed in stem cells and germ cells. Expressed at lower level in brain, heart, kidney, liver, muscle, placenta, intestine and stomach Expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen Expressed in all fetal tissues tested
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for PUM2 blocking peptide

Sequence-specific RNA-binding protein that negatively regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Capable of deadenylation-dependent and -independent modes of repression. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts.

NCBI and Uniprot Product Information

• NCBI GI #: 41688714
• NCBI GeneID: 23369
• UniProt Accession #: Q8TB72; O00234; Q53TV7; Q8WY43; Q9HAN2; B3KSL0; B4E2B6; D6W527
• Molecular Weight: 107,646 Da
• NCBI Official Full Name: Pumilio homolog 2
• NCBI Official Synonym Full Names: pumilio RNA binding family member 2
• NCBI Official Symbol: PUM2
• NCBI Official Synonym Symbols: PUMH2; PUML2
• NCBI Protein Information: pumilio homolog 2
• UniProt Protein Name: Pumilio homolog 2
• Protein Family: Pumilio
• UniProt Gene Name: PUM2
• UniProt Synonym Gene Names: KIAA0235; PUMH2; Pumilio-2
• UniProt Entry Name: PUM2_HUMAN

NCBI Description

This gene encodes a protein that belongs to a family of RNA-binding proteins. The encoded protein functions as a translational repressor during embryonic development and cell differentiation. This protein is also thought to be a positive regulator of cell proliferation in adipose-derived stem cells. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE) (, PubMed:21397187). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation (PubMed:22955276). Also mediates deadenylation-independent repression by promoting accessibility of miRNAs (PubMed:18776931, PubMed:22345517). Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3'-UTR and facilitating miRNA regulation (PubMed:22345517). Plays a role in cytoplasmic sensing of viral infection (PubMed:25340845). Represses a program of genes necessary to maintain genomic stability such as key mitotic, DNA repair and DNA replication factors. Its ability to repress those target mRNAs is regulated by the lncRNA NORAD (non-coding RNA activated by DNA damage) which, due to its high abundance and multitude of PUMILIO binding sites, is able to sequester a significant fraction of PUM1 and PUM2 in the cytoplasm (PubMed:26724866). May regulate DCUN1D3 mRNA levels (PubMed:25349211). May support proliferation and self-renewal of stem cells.