PRPF3 blocking peptide

PRPF3 Blocking Peptide

Cat. Num. AAA9626713

• Gene Names: PRPF3; PRP3; RP18; HPRP3; Prp3p; HPRP3P; SNRNP90
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: PRPF3; PRPF3 Blocking Peptide; hPrp3; HPRP3P; Pre mRNA splicing factor 3; Pre-mRNA-splicing factor 3; Precursor mRNA-processing factor 3; S. crevisiae; homolog of; PRP3 pre-mRNA processing factor 3 homolog (S. cerevisiae); Prp3p; PRPF3_HUMAN; RP18; SNRNP90; U4/U6 small nuclear ribonucleoprotein PRP3; U4/U6 snRNP 90 kDa protein; PRPF3 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for PRPF3 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for PRPF3 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 4758556
• NCBI GeneID: 9129
• NCBI Accession #: NP_004689.1
• NCBI GenBank Nucleotide #: NM_004698.3
• UniProt Accession #: O43395; O43446; Q5VT54; B4DSY9
• Molecular Weight: 31,889 Da
• NCBI Official Full Name: U4/U6 small nuclear ribonucleoprotein Prp3
• NCBI Official Synonym Full Names: pre-mRNA processing factor 3
• NCBI Official Symbol: PRPF3
• NCBI Official Synonym Symbols: PRP3; RP18; HPRP3; Prp3p; HPRP3P; SNRNP90
• NCBI Protein Information: U4/U6 small nuclear ribonucleoprotein Prp3
• UniProt Protein Name: U4/U6 small nuclear ribonucleoprotein Prp3
• Protein Family: U4/U6 small nuclear ribonucleoprotein
• UniProt Gene Name: PRPF3
• UniProt Synonym Gene Names: HPRP3; PRP3; hPrp3
• UniProt Entry Name: PRPF3_HUMAN

NCBI Description

The removal of introns from nuclear pre-mRNAs occurs on complexes called spliceosomes, which are made up of 4 small nuclear ribonucleoprotein (snRNP) particles and an undefined number of transiently associated splicing factors. This gene product is one of several proteins that associate with U4 and U6 snRNPs. Mutations in this gene are associated with retinitis pigmentosa-18. [provided by RefSeq, Jul 2008]

Uniprot Description

PRPF3: Participates in pre-mRNA splicing. May play a role in the assembly of the U4/U5/U6 tri-snRNP complex. Defects in PRPF3 are the cause of retinitis pigmentosa type 18 (RP18). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP18 inheritance is autosomal dominant.

Protein type: RNA splicing; Spliceosome; RNA processing

Chromosomal Location of Human Ortholog: 1q21.1

Cellular Component: nucleoplasm; Cajal body; spliceosome; U4/U6 x U5 tri-snRNP complex; cytoplasm; nuclear speck; nucleus

Molecular Function: identical protein binding; protein binding

Biological Process: assembly of spliceosomal tri-snRNP; nuclear mRNA splicing, via spliceosome; RNA splicing, via transesterification reactions; RNA splicing; mRNA processing

Disease: Retinitis Pigmentosa 18