POLR2L blocking peptide

POLR2L Blocking Peptide

Cat. Num. AAA9626326

• Gene Names: POLR2L; RBP10; RPB10; RPABC5; RPB7.6; hRPB7.6; RPB10beta
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: POLR2L; POLR2L Blocking Peptide; and III subunit ABC5; and III subunit RPABC5; DNA directed RNA polymerase II polypeptide L; DNA-directed RNA polymerase III subunit L; DNA-directed RNA polymerases I; hRPB7.6; hsRPB10b; II; RNA polymerase II 7.6 kDa subunit; RNA polymerase II polypeptide L; RNA polymerases I; RPAB5_HUMAN; RPABC5; RPB10; RPB10 homolog; RPB10beta; RPB7.6; POLR2L blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for POLR2L blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for POLR2L blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 10863925
• NCBI GeneID: 5441
• NCBI Accession #: NP_066951.1
• NCBI GenBank Nucleotide #: NM_021128.4
• UniProt Accession #: P62875; P52436; Q6FHX3
• Molecular Weight: 7,645 Da
• NCBI Official Full Name: DNA-directed RNA polymerases I, II, and III subunit RPABC5
• NCBI Official Synonym Full Names: polymerase (RNA) II (DNA directed) polypeptide L, 7.6kDa
• NCBI Official Symbol: POLR2L
• NCBI Official Synonym Symbols: RBP10; RPB10; RPABC5; RPB7.6; hRPB7.6; RPB10beta
• NCBI Protein Information: DNA-directed RNA polymerases I, II, and III subunit RPABC5; RPB10 homolog; RNA polymerase II 7.6 kDa subunit; DNA-directed RNA polymerase III subunit L; RNA polymerases I, II, and III subunit ABC5
• UniProt Protein Name: DNA-directed RNA polymerases I, II, and III subunit RPABC5
• UniProt Gene Name: POLR2L
• UniProt Synonym Gene Names: RNA polymerases I, II, and III subunit ABC5; RPB7.6
• UniProt Entry Name: RPAB5_HUMAN

Uniprot Description

POLR2L: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and a small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2L/RBP10 is part of the core element with the central large cleft. Belongs to the archaeal RpoN/eukaryotic RPB10 RNA polymerase subunit family.

Protein type: EC 2.7.7.6; Transcription initiation complex; Transferase; Nucleotide Metabolism - pyrimidine; DNA-binding; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 11p15

Cellular Component: nucleoplasm; DNA-directed RNA polymerase III complex; DNA-directed RNA polymerase II, core complex; nucleus; cytosol; DNA-directed RNA polymerase I complex

Molecular Function: DNA binding; zinc ion binding; DNA-directed RNA polymerase activity

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; regulation of transcription from RNA polymerase I promoter; termination of RNA polymerase III transcription; viral reproduction; positive regulation of viral transcription; somatic stem cell maintenance; RNA splicing; RNA elongation from RNA polymerase I promoter; transcription from RNA polymerase I promoter; termination of RNA polymerase I transcription; DNA repair; regulation of gene expression, epigenetic; nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase III promoter; mRNA capping; nucleotide-excision repair; negative regulation of gene expression, epigenetic; transcription-coupled nucleotide-excision repair; RNA elongation from RNA polymerase II promoter; positive regulation of interferon type I production; innate immune response; gene expression; transcription initiation from RNA polymerase I promoter; RNA elongation from RNA polymerase III promoter