POLR2E blocking peptide

POLR2E Antibody (N-term) Blocking Peptide

• Gene Names: POLR2E; RPB5; XAP4; RPABC1; hRPB25; hsRPB5
• Synonyms: POLR2E; POLR2E Antibody (N-term) Blocking Peptide; DNA-directed RNA polymerases I; II; and III subunit RPABC1; RNA polymerases I; and III subunit ABC1; DNA-directed RNA polymerase II 23 kDa polypeptide; DNA-directed RNA polymerase II subunit E; RPB5 homolog; XAP4; POLR2E blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 210

• Cellular Location: Nucleus.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for POLR2E blocking peptide

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 116242767
• NCBI GeneID: 5434
• UniProt Accession #: P19388; O43380; Q6PIH5; Q9BT06; B2R6L4; D6W5Y1
• Molecular Weight: 24,551 Da
• NCBI Official Full Name: DNA-directed RNA polymerases I, II, and III subunit RPABC1
• NCBI Official Synonym Full Names: RNA polymerase II subunit E
• NCBI Official Symbol: POLR2E
• NCBI Official Synonym Symbols: RPB5; XAP4; RPABC1; hRPB25; hsRPB5
• NCBI Protein Information: DNA-directed RNA polymerases I, II, and III subunit RPABC1
• UniProt Protein Name: DNA-directed RNA polymerases I, II, and III subunit RPABC1
• UniProt Gene Name: POLR2E
• UniProt Synonym Gene Names: RNA polymerases I, II, and III subunit ABC1
• UniProt Entry Name: RPAB1_HUMAN

NCBI Description

This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]

Uniprot Description

POLR2E: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process. Belongs to the archaeal RpoH/eukaryotic RPB5 RNA polymerase subunit family.

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cytosol; DNA-directed RNA polymerase I complex; DNA-directed RNA polymerase II, core complex; DNA-directed RNA polymerase III complex; nucleoplasm; nucleus

Molecular Function: DNA-directed RNA polymerase activity; protein binding

Biological Process: fibroblast growth factor receptor signaling pathway; gene expression; mRNA capping; nuclear mRNA splicing, via spliceosome; positive regulation of gene expression, epigenetic; positive regulation of interferon type I production; positive regulation of viral transcription; RNA elongation from RNA polymerase I promoter; RNA elongation from RNA polymerase II promoter; RNA-mediated gene silencing; snRNA transcription from RNA polymerase II promoter; somatic stem cell maintenance; termination of RNA polymerase I transcription; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase I promoter; transcription initiation from RNA polymerase II promoter; transcription-coupled nucleotide-excision repair