POLR1C blocking peptide

POLR1C Antibody (C-term) Blocking Peptide

• Gene Names: POLR1C; AC40; RPA5; TCS3; HLD11; RPA39; RPA40; RPAC1; RPC40
• Synonyms: POLR1C; POLR1C Antibody (C-term) Blocking Peptide; DNA-directed RNA polymerases I and III subunit RPAC1; DNA-directed RNA polymerase I subunit C; RNA polymerases I and III subunit AC1; AC40; DNA-directed RNA polymerases I and III 40 kDa polypeptide; RPA40; RPA39; RPC40; POLR1E; POLR1C blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human POLR1C. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 346

• Cellular Location: Nucleus.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for POLR1C blocking peptide

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. RPAC1 is part of the Pol core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 3914807
• NCBI GeneID: 9533
• UniProt Accession #: O15160; O75395; Q5JTE3
• Molecular Weight: 38,647 Da
• NCBI Official Full Name: DNA-directed RNA polymerases I and III subunit RPAC1
• NCBI Official Synonym Full Names: RNA polymerase I subunit C
• NCBI Official Symbol: POLR1C
• NCBI Official Synonym Symbols: AC40; RPA5; TCS3; HLD11; RPA39; RPA40; RPAC1; RPC40
• NCBI Protein Information: DNA-directed RNA polymerases I and III subunit RPAC1
• UniProt Protein Name: DNA-directed RNA polymerases I and III subunit RPAC1
• Protein Family: DNA-directed RNA polymerases
• UniProt Gene Name: POLR1C
• UniProt Synonym Gene Names: POLR1E; DNA-directed RNA polymerase I subunit C; RNA polymerases I and III subunit AC1; RPA40
• UniProt Entry Name: RPAC1_HUMAN

NCBI Description

The protein encoded by this gene is a subunit of both RNA polymerase I and RNA polymerase III complexes. The encoded protein is part of the Pol core element. Mutations in this gene have been associated with Treacher Collins syndrome (TCS) and hypomyelinating leukodystrophy 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

Uniprot Description

RPA40: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. RPAC1 is part of the Pol core element with the central large cleft and probably a clamp element that moves to open and close the cleft. Defects in POLR1C are the cause of Treacher Collins syndrome type 3 (TCS3). A form of Treacher Collins syndrome, a disorder of craniofacial development. Treacher Collins syndrome is characterized by a combination of bilateral downward slanting of the palpebral fissures, colobomas of the lower eyelids with a paucity of eyelashes medial to the defect, hypoplasia of the facial bones, cleft palate, malformation of the external ears, atresia of the external auditory canals, and bilateral conductive hearing loss. Belongs to the archaeal RpoD/eukaryotic RPB3 RNA polymerase subunit family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.7.6; Nucleotide Metabolism - purine; Transcription initiation complex; Nucleotide Metabolism - pyrimidine; Transferase

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: cytosol; DNA-directed RNA polymerase III complex; nucleoplasm

Molecular Function: DNA-directed RNA polymerase activity; protein binding

Biological Process: positive regulation of gene expression, epigenetic; positive regulation of interferon type I production; RNA elongation from RNA polymerase I promoter; termination of RNA polymerase I transcription; transcription from RNA polymerase I promoter; transcription initiation from RNA polymerase I promoter

Disease: Leukodystrophy, Hypomyelinating, 11; Treacher Collins Syndrome 3