POLR1A blocking peptide

POLR1A Antibody (C-term) Blocking Peptide

• Gene Names: POLR1A; A190; RPA1; RPO14; AFDCIN; RPA194; RPO1-4
• Synonyms: POLR1A; POLR1A Antibody (C-term) Blocking Peptide; DNA-directed RNA polymerase I subunit RPA1; RNA polymerase I subunit A1; A190; DNA-directed RNA polymerase I largest subunit; DNA-directed RNA polymerase I subunit A; RNA polymerase I 194 kDa subunit; RPA194; POLR1A blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 1720

• Cellular Location: Nucleus, nucleolus.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for POLR1A blocking peptide

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 215273873
• NCBI GeneID: 25885
• UniProt Accession #: O95602; Q0VG05; Q9UEH0; Q9UFT9; B7Z7T0; D6W5M0
• Molecular Weight: 194,811 Da
• NCBI Official Full Name: DNA-directed RNA polymerase I subunit RPA1
• NCBI Official Synonym Full Names: RNA polymerase I subunit A
• NCBI Official Symbol: POLR1A
• NCBI Official Synonym Symbols: A190; RPA1; RPO14; AFDCIN; RPA194; RPO1-4
• NCBI Protein Information: DNA-directed RNA polymerase I subunit RPA1
• UniProt Protein Name: DNA-directed RNA polymerase I subunit RPA1
• UniProt Gene Name: POLR1A
• UniProt Synonym Gene Names: RNA polymerase I subunit A1; RPA194
• UniProt Entry Name: RPA1_HUMAN

NCBI Description

The protein encoded by this gene is the largest subunit of the RNA polymerase I complex. The encoded protein represents the catalytic subunit of the complex, which transcribes DNA into ribosomal RNA precursors. Defects in this gene are a cause of the Cincinnati type of acrofacial dysostosis. [provided by RefSeq, May 2016]

Uniprot Description

POLR1A: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. Belongs to the RNA polymerase beta' chain family.

Protein type: Nucleolus; Transcription initiation complex; Nucleotide Metabolism - purine; Transferase; EC 2.7.7.6; Nucleotide Metabolism - pyrimidine

Chromosomal Location of Human Ortholog: 2p11.2

Cellular Component: cytoplasm; DNA-directed RNA polymerase I complex; nucleolus; nucleoplasm; nucleus

Molecular Function: protein binding

Biological Process: positive regulation of gene expression, epigenetic; RNA elongation from RNA polymerase I promoter; termination of RNA polymerase I transcription; transcription initiation from RNA polymerase I promoter

Disease: Acrofacial Dysostosis, Cincinnati Type